Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154412_47jb AGAATTCAATATAGTTGTTGCTGTGAATATAG(+CAGTGT)GTACCATTTTGGGTCATCTACACTAAGACTG NGG 10 8 17 8 24 0 100 5 10
    240605154411_j3bt TGTGTCAGTCTTAGTGTAGATGACCCAAAATG(+CAGTGT)GTACCTATATTCACAGCAACAACTATATTGA NGG 10 8 17 8 24 0 100 5 10
    240605154410_757m TAGTTTATCACATTGAAATAAGAATCTCTTTG(+CAGTGT)GTACCTTTTATTCAGTAAATCTTAACAATGA NGG 10 8 17 8 24 0 100 5 10
    240605154408_ryuu TTCTGTCATTGTTAAGATTTACTGAATAAAAG(+CAGTGT)GTACCAAAGAGATTCTTATTTCAATGTGATA NGG 10 8 17 8 24 0 100 5 10
    240605154407_nmes CAAGTGGAATGACTAAATGACCCAGCTGTTTG(+CAGTGT)GATCCAGGCAGCTTGGGTATCCATGGGGTTA NGG 10 8 17 8 24 0 100 5 10
    240605154405_jx4w CAGGCTAACCCCATGGATACCCAAGCTGCCTG(+CAGTGT)GATCCAAACAGCTGGGTCATTTAGTCATTCC NGG 10 8 17 8 24 0 100 5 10
    240605154403_2u1w AACTTTTGGGTTTGAGCTACAAACTACACCAG(+CAGTGT)GTACCACCCCAGATAGAGGGTGTCTTGGGTG NGG 10 8 17 8 24 0 100 5 10
    240605154401_rozc GCAGTCACCCAAGACACCCTCTATCTGGGGTG(+CAGTGT)GTACCTGGTGTAGTTTGTAGCTCAAACCCAA NGG 10 8 17 8 24 0 100 5 10
    240605154400_5xkm CTGTGTGTATGTGCATGCAAACTCAGAGGAAG(+CAGTGT)GTACCAGGCTCCCTGGGACTAGAGTTATGGA NGG 10 8 17 8 24 0 100 5 10
    240605154358_qiw3 CGCCATCCATAACTCTAGTCCCAGGGAGCCTG(+CAGTGT)GTACCTTCCTCTGAGTTTGCATGCACATACA NGG 10 8 17 8 24 0 100 5 10