Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154503_8t01 GGACCTTTATTTACCTAACTTAAGATCATTAG(+CAGTGT)GTACCTGGTTTGGCTTACACTCAAACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605154501_15ds GCAGACCTGGGTTTGAGTGTAAGCCAAACCAG(+CAGTGT)GTACCTAATGATCTTAAGTTAGGTAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240605154500_un5u GTTGGGGGTGGGGTGTTACATGCATGTCACTG(+CAGTGT)GATCCTATGGAAGTTGTGAGCTGCCATGTAG NGG 10 8 17 8 24 0 100 5 10
    240605154458_ag66 AGCAGCTACATGGCAGCTCACAACTTCCATAG(+CAGTGT)GATCCAGTGACATGCATGTAACACCCCACCC NGG 10 8 17 8 24 0 100 5 10
    240605154456_nqfg AGAGGGCCAATGAAACCCAAAGGTGAGAAAGG(+CAGTGT)GATCCTGGGCACAAGTGAGATAAACTTAATC NGG 10 8 17 8 24 0 100 5 10
    240605154454_uho8 CTTAGGATTAAGTTTATCTCACTTGTGCCCAG(+CAGTGT)GATCCCTTTCTCACCTTTGGGTTTCATTGGC NGG 10 8 17 8 24 0 100 5 10
    240605154452_4yx4 GCAGTTTTGTGGCAGAATGGGCCATTGTTGTG(+CAGTGT)GATCCATCCAAACGTATCCCCAGCTAAAATA NGG 10 8 17 8 24 0 100 5 10
    240605154451_2eg1 TCTATTATTTTAGCTGGGGATACGTTTGGATG(+CAGTGT)GATCCACAACAATGGCCCATTCTGCCACAAA NGG 10 8 17 8 24 0 100 5 10
    240605154448_hfi0 AAAATGTCCTTGATAATAAATGGTATGGCCCG(+CAGTGT)GATCCTATTTTGATAAGATCCAGGGGAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605154447_knqm ACAGATAGCTCCCCTGGATCTTATCAAAATAG(+CAGTGT)GATCCGGGCCATACCATTTATTATCAAGGAC NGG 10 8 17 8 24 0 100 5 10