Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154535_vh9j TTCATTTTAGATTACTAATAAAGGGAGAAAAG(+CAGTGT)GTACCTTCAAAGCTTATTCACATTTGCAAAA NGG 10 8 17 8 24 0 100 5 10
    240605154534_uote AAAGATTTTGCAAATGTGAATAAGCTTTGAAG(+CAGTGT)GTACCTTTTCTCCCTTTATTAGTAATCTAAA NGG 10 8 17 8 24 0 100 5 10
    240605154532_vzm9 TCATTTCCCAGAGGGCGTCAGATGGTGGTATG(+CAGTGT)GATCCTGCCTCCTGAACTCAATCCTTTCAGT NGG 10 8 17 8 24 0 100 5 10
    240605154531_w09b CCTGCACTGAAAGGATTGAGTTCAGGAGGCAG(+CAGTGT)GATCCATACCACCATCTGACGCCCTCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605154529_5aqh AGTCATTGTTAGGTGTTCGTGTTGGGAATGGG(+CAGTGT)GATCCTTCAAGGAACAGAGTAAAGCACCTTC NGG 10 8 17 8 24 0 100 5 10
    240605154528_8y4t AGGGTGAAGGTGCTTTACTCTGTTCCTTGAAG(+CAGTGT)GATCCCCATTCCCAACACGAACACCTAACAA NGG 10 8 17 8 24 0 100 5 10
    240605154527_pxrr TACACCGGGTCTCTTTAAAATTGGCATGCTCG(+CAGTGT)GTACCAAATAGGTGAGCTCATAATTAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240605154525_1it7 CTGTAATTTCTAATTATGAGCTCACCTATTTG(+CAGTGT)GTACCGAGCATGCCAATTTTAAAGAGACCCG NGG 10 8 17 8 24 0 100 5 10
    240605154524_k6ki ATCCAGAGCAGTGATGATGAGCAGGTAGACGG(+CAGTGT)GTACCAGCCGGCGGGCTTCTGGATAGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240605154522_wx8s ACAGGGACCCCTATCCAGAAGCCCGCCGGCTG(+CAGTGT)GTACCCGTCTACCTGCTCATCATCACTGCTC NGG 10 8 17 8 24 0 100 5 10