Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605155022_ofp2 TCTCTAGAGTTCTGGAGAGGGATAGAATGAAG(+CAGTGT)GATCCCAAGGAATTGGAGTAAAGGTTTTGTG NGG 10 8 17 8 24 0 100 5 10
    240605155021_q4is GCAGCAGGCAGGTGGAAGGTAACCACTGTCAG(+CAGTGT)GATCCCCTGAACTGCACGTGTCCTTCCCTAC NGG 10 8 17 8 24 0 100 5 10
    240605155019_yzpp CCAAAGTAGGGAAGGACACGTGCAGTTCAGGG(+CAGTGT)GATCCTGACAGTGGTTACCTTCCACCTGCCT NGG 10 8 17 8 24 0 100 5 10
    240605155018_371c AGATATGCTTTAAAGAGAGGCCTTAATTTAAG(+CAGTGT)GTACCGCCATCTTGGCTCACAAGAGCCCAGT NGG 10 8 17 8 24 0 100 5 10
    240605155016_gxj9 TCTGAACTGGGCTCTTGTGAGCCAAGATGGCG(+CAGTGT)GTACCTTAAATTAAGGCCTCTCTTTAAAGCA NGG 10 8 17 8 24 0 100 5 10
    240605155014_sk8f GTGACACATTGAAGAAGATGAGTGAGATCCAG(+CAGTGT)GATCCCCACTCTATCCACACATCCAAGATCA NGG 10 8 17 8 24 0 100 5 10
    240605155012_ilr2 CCAGTTGATCTTGGATGTGTGGATAGAGTGGG(+CAGTGT)GATCCTGGATCTCACTCATCTTCTTCAATGT NGG 10 8 17 8 24 0 100 5 10
    240605155010_9r29 CGAGGAAGAACTATGTCAAAGGCTGGCTAGGG(+CAGTGT)GTACCCAGCACCGTAGCCTTGCCACCTGCCA NGG 10 8 17 8 24 0 100 5 10
    240605155008_qm2n TCTGCTGGCAGGTGGCAAGGCTACGGTGCTGG(+CAGTGT)GTACCCCTAGCCAGCCTTTGACATAGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240605155007_ov1k GCTTTTCAACTGACTTCCTCTGGAGTGGGGAG(+CAGTGT)GTACCAACTGATTTCCAGAAGGCTAAAGCAC NGG 10 8 17 8 24 0 100 5 10