Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605155111_aclx TAGAGAGCCACAGGAGCCAGGTGCTAGAGAGG(+CAGTGT)GATCCCTCCCCACCCCCAGATTGCTGACTGT NGG 10 8 17 8 24 0 100 5 10
    240605155109_lywi GGCAAAGACTGAGCTCAGGCACCAGCCTCCCG(+CAGTGT)GTACCACGCTGGAGGTGGATCCCTGAGTAGA NGG 10 8 17 8 24 0 100 5 10
    240605155108_sl46 TGCCTTCTACTCAGGGATCCACCTCCAGCGTG(+CAGTGT)GTACCGGGAGGCTGGTGCCTGAGCTCAGTCT NGG 10 8 17 8 24 0 100 5 10
    240605155106_1ezi GGCACCAGCCTCCCGGTACCACGCTGGAGGTG(+CAGTGT)GATCCCTGAGTAGAAGGCAGGGTAAGCTTCG NGG 10 8 17 8 24 0 100 5 10
    240605155105_t4xq GAAGTCGAAGCTTACCCTGCCTTCTACTCAGG(+CAGTGT)GATCCACCTCCAGCGTGGTACCGGGAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605155103_ekh3 CTGTGCCAGAGCCGCTAGCAGCTGCTCACAGG(+CAGTGT)GTACCTTTGTGGGTCTGGTGTTTTGAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240605155102_udp7 GGGTGATTTCTCAAAACACCAGACCCACAAAG(+CAGTGT)GTACCCTGTGAGCAGCTGCTAGCGGCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605155059_wawl GAGACGAGGGGCGGGAGGTCAGCTTCTCCCTG(+CAGTGT)GATCCTCAGTGTGCACTGCCTTGGGCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605155058_jf5a GTGGCTCCCTGCCCAAGGCAGTGCACACTGAG(+CAGTGT)GATCCAGGGAGAAGCTGACCTCCCGCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240605155057_a9jg GACCCGCGGCTTGCGCATCTGTGACATTTCTG(+CAGTGT)GATCCCGGTCAGTTCCGGCTGTGCCCAGACT NGG 10 8 17 8 24 0 100 5 10