Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605155558_dg4g TGGTCCTGCCCTTGTCGAAGGGAGGTTGGCAG(+CAGTGT)GTACCCCAGAGATCAGGGGAGATGCATGAAA NGG 10 8 17 8 24 0 100 5 10
    240605155556_wy8v AGCACATGTGTGTGCATGTGTGTGTATGCCTG(+CAGTGT)GATCCATGCCAGCGACTGGGCATTGGAGGGT NGG 10 8 17 8 24 0 100 5 10
    240605155555_brz3 CCTAGACCCTCCAATGCCCAGTCGCTGGCATG(+CAGTGT)GATCCAGGCATACACACACATGCACACACAT NGG 10 8 17 8 24 0 100 5 10
    240605155553_zxlp GACTTAGGGTATAGGACATACGCCATGACAGG(+CAGTGT)GATCCTATAAGGAAAACGTGCTAGACATCAG NGG 10 8 17 8 24 0 100 5 10
    240605155552_v5fr GTTTGCTGATGTCTAGCACGTTTTCCTTATAG(+CAGTGT)GATCCCTGTCATGGCGTATGTCCTATACCCT NGG 10 8 17 8 24 0 100 5 10
    240605155551_8f7t CATCTGTGCTCCATTGCTCCAAAAGCCAGCAG(+CAGTGT)GTACCTGAGGGCTAGTGTCTGAACTCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240605155549_1f4f TGAAAGCTGAGAGTTCAGACACTAGCCCTCAG(+CAGTGT)GTACCTGCTGGCTTTTGGAGCAATGGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605155548_lr4b GAGGAACCTTCAACGTAGGCAGCTATTAAATG(+CAGTGT)GATCCAGTATTTCTCAGAGCATCCTATGCAA NGG 10 8 17 8 24 0 100 5 10
    240605155546_lgtu TCACCTTGCATAGGATGCTCTGAGAAATACTG(+CAGTGT)GATCCATTTAATAGCTGCCTACGTTGAAGGT NGG 10 8 17 8 24 0 100 5 10
    240605155545_eego TGAGTGACCTGGGATACATAGACACCCTTATG(+CAGTGT)GATCCTTGACATCTGTGCTCCATTGCTCCAA NGG 10 8 17 8 24 0 100 5 10