Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605133710_w7oc ATGCAACACTGGGTGTTCACCTGCCTTACTGG(+CAGTGT)GATCCCATGTGTTTAGTAATTTAACGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605133709_oelj TTCAGCTGGACGTTAAATTACTAAACACATGG(+CAGTGT)GATCCCAGTAAGGCAGGTGAACACCCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605133707_f0nh AGACTGCTGGGAAACCCAGGCCAAAGGGCAGG(+CAGTGT)GATCCAGACTGGCCATCTCATTGTACACTGT NGG 10 8 17 8 24 0 100 5 10
    240605133706_arj2 ATGACACAGTGTACAATGAGATGGCCAGTCTG(+CAGTGT)GATCCCTGCCCTTTGGCCTGGGTTTCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605133704_azi1 GCATGGATTTGATCATGGGGGCCAGGGGCCAG(+CAGTGT)GTACCCCTGACCCTCTCAGTGTGGAGAGATG NGG 10 8 17 8 24 0 100 5 10
    240605133702_67h9 AGGTGCATCTCTCCACACTGAGAGGGTCAGGG(+CAGTGT)GTACCTGGCCCCTGGCCCCCATGATCAAATC NGG 10 8 17 8 24 0 100 5 10
    240605133701_1y5v ACTTGCTTTTTTTTTTTTTTAATTTGATGCTG(+CAGTGT)GATCCAGAATAGGCCAGTGAAGATCAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605133659_09ty ACCACTGCTCTGATCTTCACTGGCCTATTCTG(+CAGTGT)GATCCAGCATCAAATTAAAAAAAAAAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605133658_ptdn TAACTTGAACTTGCAGGTCACCTCTGAACAGG(+CAGTGT)GTACCCTTGTCCCCACCCCAGGCCCTGTTAC NGG 10 8 17 8 24 0 100 5 10
    240605133656_wrko ACAGGGTAACAGGGCCTGGGGTGGGGACAAGG(+CAGTGT)GTACCCTGTTCAGAGGTGACCTGCAAGTTCA NGG 10 8 17 8 24 0 100 5 10