Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605133611_5h0f TACAGACCCAGTCTCCAGCTGTTTTGACACTG(+CAGTGT)GATCCTTGTAAAACTGTACAAGTCATTTGTA NGG 10 8 17 8 24 0 100 5 10
    240605133609_9iuc TGAATTACAAATGACTTGTACAGTTTTACAAG(+CAGTGT)GATCCAGTGTCAAAACAGCTGGAGACTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605133608_3ezn GCTGGAGAGCTGCTCACACAGCTGGACTTCTG(+CAGTGT)GATCCTTACACTCACACCCGCTTTCATTTGG NGG 10 8 17 8 24 0 100 5 10
    240605133607_uu5b AAGTACCAAATGAAAGCGGGTGTGAGTGTAAG(+CAGTGT)GATCCAGAAGTCCAGCTGTGTGAGCAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605133606_s499 TATTTTCAAATGTGTTCCTCCCTGGAAAATAG(+CAGTGT)GATCCTTCTTACACAAACCTGTATTTTATAG NGG 10 8 17 8 24 0 100 5 10
    240605133604_objb TTTTTCTATAAAATACAGGTTTGTGTAAGAAG(+CAGTGT)GATCCTATTTTCCAGGGAGGAACACATTTGA NGG 10 8 17 8 24 0 100 5 10
    240605133603_eqhp CGTGACATGGGTTAGGCTCGAACTGCACAGTG(+CAGTGT)GTACCAATGTGGTCTGGCCTTGAACAACTCA NGG 10 8 17 8 24 0 100 5 10
    240605133602_io0x CTTAGTGAGTTGTTCAAGGCCAGACCACATTG(+CAGTGT)GTACCACTGTGCAGTTCGAGCCTAACCCATG NGG 10 8 17 8 24 0 100 5 10
    240605133601_a112 AAAAAAAATCACACGTGGAAGAAAAGAAGTCG(+CAGTGT)GTACCAGTACAAAAAGACTGCAGGGCCTACT NGG 10 8 17 8 24 0 100 5 10
    240605133600_zg1x CTGACAGTAGGCCCTGCAGTCTTTTTGTACTG(+CAGTGT)GTACCGACTTCTTTTCTTCCACGTGTGATTT NGG 10 8 17 8 24 0 100 5 10