Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605133653_2kd0 TCTCCTGTGTAGACTAGCTGGAGGTCCCTGGG(+CAGTGT)GATCCCCAGGGAAAGGACGCTCTGCCGCGCA NGG 10 8 17 8 24 0 100 5 10
    240605133651_bpys GAGATTGCGCGGCAGAGCGTCCTTTCCCTGGG(+CAGTGT)GATCCCCAGGGACCTCCAGCTAGTCTACACA NGG 10 8 17 8 24 0 100 5 10
    240605133649_o6cc CTTTGTCCTCGTCGAGCTGCTCTGAGCTGTGG(+CAGTGT)GATCCTGTGAACAACTGTCGTCTTTCCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605133648_l58i TGAAGAGAAGGAAAGACGACAGTTGTTCACAG(+CAGTGT)GATCCCACAGCTCAGAGCAGCTCGACGAGGA NGG 10 8 17 8 24 0 100 5 10
    240605133647_y8o2 CCAAAATGCCCGTCCTATAGAGTGTCTGATTG(+CAGTGT)GATCCTCTGTTGCGTATTCTGATGGTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605133646_75f2 ACCAGCAGGCACCATCAGAATACGCAACAGAG(+CAGTGT)GATCCAATCAGACACTCTATAGGACGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605133645_owok GCTGTTGTCAGTCTCCATGAAAGCTCTAGCTG(+CAGTGT)GTACCTTGGGTCTTGTCCTGACACCCGTATC NGG 10 8 17 8 24 0 100 5 10
    240605133642_jbxo TCACAGATACGGGTGTCAGGACAAGACCCAAG(+CAGTGT)GTACCAGCTAGAGCTTTCATGGAGACTGACA NGG 10 8 17 8 24 0 100 5 10
    240605133641_mwa1 GTGTCTCATAAAGCAGCTGTTCAGGATGTGTG(+CAGTGT)GATCCTCTGCCATTGCTCTCCCGCGTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605133640_nuni GAGCTTCCCCACGCGGGAGAGCAATGGCAGAG(+CAGTGT)GATCCACACATCCTGAACAGCTGCTTTATGA NGG 10 8 17 8 24 0 100 5 10