Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132732_g5mw CTATATCAAGCAGAACACAGGTCATCAGCTTG(+CAGTGT)GATCCATGATTTGACTTCAAGTCATTCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605132730_obix CAGTGCAAAGAATGACTTGAAGTCAAATCATG(+CAGTGT)GATCCAAGCTGATGACCTGTGTTCTGCTTGA NGG 10 8 17 8 24 0 100 5 10
    240605132728_wvx4 GCTGGGGGTACTTGCCATGAAAATTCCCTTGG(+CAGTGT)GATCCTGTGACCCTCTGGGGACAGAAGCACA NGG 10 8 17 8 24 0 100 5 10
    240605132727_19bx ATGTGTGTGCTTCTGTCCCCAGAGGGTCACAG(+CAGTGT)GATCCCAAGGGAATTTTCATGGCAAGTACCC NGG 10 8 17 8 24 0 100 5 10
    240605132725_4snu AATCTTGCGGAGTTCCAGAACCATACTGTGAG(+CAGTGT)GTACCACAGGGTCCTGTGGCCCAGTAGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605132723_p7vh AGAACAAAACTACTGGGCCACAGGACCCTGTG(+CAGTGT)GTACCTCACAGTATGGTTCTGGAACTCCGCA NGG 10 8 17 8 24 0 100 5 10
    240605132722_wdbd GGGAATCAATTATTTTCTCCTTTAGAATTTAG(+CAGTGT)GATCCAAGTAAGATCATACAGGGGTGTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605132721_3l5z AGTTGGGACACACCCCTGTATGATCTTACTTG(+CAGTGT)GATCCTAAATTCTAAAGGAGAAAATAATTGA NGG 10 8 17 8 24 0 100 5 10
    240605132719_z29c ATTTATACTTTTTAAATAAATGGTACTTGCTG(+CAGTGT)GATCCACACTAACATCTTTGCTAACAATCCC NGG 10 8 17 8 24 0 100 5 10
    240605132718_vi51 ACAATGGGATTGTTAGCAAAGATGTTAGTGTG(+CAGTGT)GATCCAGCAAGTACCATTTATTTAAAAAGTA NGG 10 8 17 8 24 0 100 5 10