Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132818_97lf TAGGCTATATACCATGTTTGCAGTCAGCCTGG(+CAGTGT)GATCCATGAAACCCTGTCTCAAGAAAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240605132817_q9kq TATTTTTGCTTTTCTTGAGACAGGGTTTCATG(+CAGTGT)GATCCCAGGCTGACTGCAAACATGGTATATA NGG 10 8 17 8 24 0 100 5 10
    240605132816_w5pc CTAGGGGCTGTGGAGGCTGGAGGAGAGGATCG(+CAGTGT)GATCCCTTAAATACCCATGTGGATGCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605132814_6a74 TCAGTTCCCAGCATCCACATGGGTATTTAAGG(+CAGTGT)GATCCGATCCTCTCCTCCAGCCTCCACAGCC NGG 10 8 17 8 24 0 100 5 10
    240605132813_s1a7 AAGTCCTTGCTCTTAGCCAGACTAGAGATTAG(+CAGTGT)GATCCCACATGTTTATGCTGTTTGTTTTTGG NGG 10 8 17 8 24 0 100 5 10
    240605132811_f9yz CTATCCCAAAAACAAACAGCATAAACATGTGG(+CAGTGT)GATCCTAATCTCTAGTCTGGCTAAGAGCAAG NGG 10 8 17 8 24 0 100 5 10
    240605132809_r47i TAGGTCTTACACTGACTTCTTGTGTCTCCCAG(+CAGTGT)GATCCTTGTTGTTATTAATGTCACACAACAG NGG 10 8 17 8 24 0 100 5 10
    240605132807_fnq0 TAAAACTGTTGTGTGACATTAATAACAACAAG(+CAGTGT)GATCCTGGGAGACACAAGAAGTCAGTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240605132806_d0b6 AACACGGGCAGTCCCAGTCCGCGTGGGCGTGG(+CAGTGT)GATCCCGAGTGTGGGGAACTGTGTGGGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605132804_3yzq CCACTTGGACCCACACAGTTCCCCACACTCGG(+CAGTGT)GATCCCACGCCCACGCGGACTGGGACTGCCC NGG 10 8 17 8 24 0 100 5 10