Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132921_z76r AAATAGGGAAAGAAGTCAGTGGCTGGTAGAGG(+CAGTGT)GTACCACTCCCCAGATGCTGTGTAGTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240605132920_j05s CAGGGCCTCAACTACACAGCATCTGGGGAGTG(+CAGTGT)GTACCCTCTACCAGCCACTGACTTCTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605132918_9be0 CTAAGCAGCCAATGTCGCTTCTGGGGCTCCTG(+CAGTGT)GATCCTCTAGGCGGAGTCCTTTATGGTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605132917_xd23 GGGGGGGACACCATAAAGGACTCCGCCTAGAG(+CAGTGT)GATCCAGGAGCCCCAGAAGCGACATTGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605132915_zeeu CTTTCCTTCCACACTGTCTTGTATCATGGCTG(+CAGTGT)GATCCAGTGTATTTCCAGCTAATTGTTAATG NGG 10 8 17 8 24 0 100 5 10
    240605132914_41ks CATCACATTAACAATTAGCTGGAAATACACTG(+CAGTGT)GATCCAGCCATGATACAAGACAGTGTGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605132913_i35g TATAATTTCTGTTGACTTCAAGCTTTAATTTG(+CAGTGT)GATCCACACCTACAGCTGACCCAAATGTCTC NGG 10 8 17 8 24 0 100 5 10
    240605132912_m1wy CAGAGGAGACATTTGGGTCAGCTGTAGGTGTG(+CAGTGT)GATCCAAATTAAAGCTTGAAGTCAACAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605132910_frwz TTGGGACATTGGTTACATGGGTTATTGTATAG(+CAGTGT)GATCCTAAAATACATGTGGGGAAGAGTAATT NGG 10 8 17 8 24 0 100 5 10
    240605132909_9k8f TTTGAAATTACTCTTCCCCACATGTATTTTAG(+CAGTGT)GATCCTATACAATAACCCATGTAACCAATGT NGG 10 8 17 8 24 0 100 5 10