Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132934_juu2 GGAGATTCATCAGAACTCAGCACACGCTGGTG(+CAGTGT)GATCCACAGCACTGTCATGTCTGTTATGAAT NGG 10 8 17 8 24 0 100 5 10
    240605132933_fgf2 GTGTCATTCATAACAGACATGACAGTGCTGTG(+CAGTGT)GATCCACCAGCGTGTGCTGAGTTCTGATGAA NGG 10 8 17 8 24 0 100 5 10
    240605132932_deth CCTAACCCATGCGCTGCTGGTGGGTACTGAAG(+CAGTGT)GTACCCACTTGGACTTGGAGGGGTAGGCTCC NGG 10 8 17 8 24 0 100 5 10
    240605132930_nw5x GTCATGGAGCCTACCCCTCCAAGTCCAAGTGG(+CAGTGT)GTACCTTCAGTACCCACCAGCAGCGCATGGG NGG 10 8 17 8 24 0 100 5 10
    240605132929_e8wf TTACTTTGCGTGGCTGGGAGAAGAGTAGACTG(+CAGTGT)GATCCTGACCTTTCTGCACACTTCCAGAACC NGG 10 8 17 8 24 0 100 5 10
    240605132928_u6f9 GCGTTGGTTCTGGAAGTGTGCAGAAAGGTCAG(+CAGTGT)GATCCAGTCTACTCTTCTCCCAGCCACGCAA NGG 10 8 17 8 24 0 100 5 10
    240605132926_qp30 GTTCTGCCAAGACCGGCAGGGTAAACAAACAG(+CAGTGT)GTACCACTCTCTCCCGGGAACCCTTTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605132925_3296 ACAACAGAAGAAAGGGTTCCCGGGAGAGAGTG(+CAGTGT)GTACCTGTTTGTTTACCCTGCCGGTCTTGGC NGG 10 8 17 8 24 0 100 5 10
    240605132924_7xhu CAGGAAAGGTTCCGTTTATAAACAAGAAACTG(+CAGTGT)GTACCAGAAAATTGCAGAGAAGCACCAACGT NGG 10 8 17 8 24 0 100 5 10
    240605132923_kmz6 GGAGCACGTTGGTGCTTCTCTGCAATTTTCTG(+CAGTGT)GTACCAGTTTCTTGTTTATAAACGGAACCTT NGG 10 8 17 8 24 0 100 5 10