Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132835_c2bw GCCTGACTTTTTCCTGTTTTGAGCCATCTGTG(+CAGTGT)GTACCTGTCCAAGGTGGTAACTCCACTATAG NGG 10 8 17 8 24 0 100 5 10
    240605132834_hs3h CACTACTATAGTGGAGTTACCACCTTGGACAG(+CAGTGT)GTACCACAGATGGCTCAAAACAGGAAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605132832_zgp0 TGGACTGTCCACTAGAGTTGGACAGTACTGGG(+CAGTGT)GTACCCAGGTCCACCCCTTGGAATACCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605132830_sgpl TCCAGACAGGGTATTCCAAGGGGTGGACCTGG(+CAGTGT)GTACCCCAGTACTGTCCAACTCTAGTGGACA NGG 10 8 17 8 24 0 100 5 10
    240605132828_ybnq TAGCAAGCTCACTCACCAAGCCGCACTGGGTG(+CAGTGT)GATCCATCTTTGGTTTGTTGGAGAGAACAGA NGG 10 8 17 8 24 0 100 5 10
    240605132827_803i TAGAGTCTGTTCTCTCCAACAAACCAAAGATG(+CAGTGT)GATCCACCCAGTGCGGCTTGGTGAGTGAGCT NGG 10 8 17 8 24 0 100 5 10
    240605132825_s2ws TTGTTTCCTCCCAAATACTCCCTGAACGGGAG(+CAGTGT)GATCCCCTTCTGCTCCATCAGGACTCCACTG NGG 10 8 17 8 24 0 100 5 10
    240605132823_7k2o GGCTTCAGTGGAGTCCTGATGGAGCAGAAGGG(+CAGTGT)GATCCTCCCGTTCAGGGAGTATTTGGGAGGA NGG 10 8 17 8 24 0 100 5 10
    240605132822_6you CCCACCACGCTCCCTAGCTGGGAGAGAAACAG(+CAGTGT)GATCCACAAGAGGAGTGGGAAAGCCGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605132821_gicz GTGTCAAAAACGGCTTTCCCACTCCTCTTGTG(+CAGTGT)GATCCTGTTTCTCTCCCAGCTAGGGAGCGTG NGG 10 8 17 8 24 0 100 5 10