Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605133041_q7pz TTCTCCCCACAGCCACAGGGAAGTTGACACTG(+CAGTGT)GATCCCTCTGCTTGTCCCCATCTTTTTTGCT NGG 10 8 17 8 24 0 100 5 10
    240605133039_sbn7 AGGTGAGCAAAAAAGATGGGGACAAGCAGAGG(+CAGTGT)GATCCAGTGTCAACTTCCCTGTGGCTGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605133038_d6m8 AGCCAACAGACTTGAAAAGGCCCCTTGTCTAG(+CAGTGT)GTACCCCACGAAAATAAACTTTGAAAACGTA NGG 10 8 17 8 24 0 100 5 10
    240605133036_2z1w CTGGATACGTTTTCAAAGTTTATTTTCGTGGG(+CAGTGT)GTACCTAGACAAGGGGCCTTTTCAAGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240605133035_qnaj GAGAACAGAAGCGCCTGGGGAAGAAGGGTGAG(+CAGTGT)GATCCCCCAAATCAGAGTATGCCTGGGAGTG NGG 10 8 17 8 24 0 100 5 10
    240605133032_hre5 TCAATCACTCCCAGGCATACTCTGATTTGGGG(+CAGTGT)GATCCTCACCCTTCTTCCCCAGGCGCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605133030_cotm GGGATGGGCTTAGACCATTAAAGGGACCAGGG(+CAGTGT)GATCCTGGCATGTGCAAGTGGTGATGGCCGT NGG 10 8 17 8 24 0 100 5 10
    240605133028_lp79 AACCCACGGCCATCACCACTTGCACATGCCAG(+CAGTGT)GATCCCCTGGTCCCTTTAATGGTCTAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240605133027_x2vn GGAAAATGTCTCCGTGGATCACTCAGTGATAG(+CAGTGT)GATCCGCCTCTTGAGTGCTGTCAGTGGCTAG NGG 10 8 17 8 24 0 100 5 10
    240605133025_m84p CTGAACTAGCCACTGACAGCACTCAAGAGGCG(+CAGTGT)GATCCTATCACTGAGTGATCCACGGAGACAT NGG 10 8 17 8 24 0 100 5 10