Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605133057_bxve TTTAATCTTTTCCCTCTGTCAGAGTACCCAGG(+CAGTGT)GATCCATGAAGTGCCTGTCGCTCGAAGACTC NGG 10 8 17 8 24 0 100 5 10
    240605133056_eb0m GCCTGGAGTCTTCGAGCGACAGGCACTTCATG(+CAGTGT)GATCCCTGGGTACTCTGACAGAGGGAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605133055_ap2i CCCACTTCTCAGGAATGACCTTCCACAGCTGG(+CAGTGT)GTACCCCTTAAATCTGCTATGTTCCTGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605133052_zwoo ACCTGGAGGCAGGAACATAGCAGATTTAAGGG(+CAGTGT)GTACCCAGCTGTGGAAGGTCATTCCTGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605133051_sihi GTGTAACCACGGCCACCTCATTGCAGAGCAGG(+CAGTGT)GATCCAGGTTAGGGAAGTATAGAGTCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240605133049_m3y5 TCTGTTCTGGGACTCTATACTTCCCTAACCTG(+CAGTGT)GATCCCTGCTCTGCAATGAGGTGGCCGTGGT NGG 10 8 17 8 24 0 100 5 10
    240605133047_wjlz GGCACAGTGGTAGAGCCACTCTGGATTGATGG(+CAGTGT)GATCCAATGGAATGACCTAGAGCTAAATGCA NGG 10 8 17 8 24 0 100 5 10
    240605133045_axl8 GGGGTTGCATTTAGCTCTAGGTCATTCCATTG(+CAGTGT)GATCCCATCAATCCAGAGTGGCTCTACCACT NGG 10 8 17 8 24 0 100 5 10
    240605133043_tuyf TTTGCTCACCTGGGCAAGAAGTCATTTCCTGG(+CAGTGT)GTACCTTTCTAGCCCTGCCCATCCCAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605133042_29ju AGAAGGAGGCTGGGATGGGCAGGGCTAGAAAG(+CAGTGT)GTACCCAGGAAATGACTTCTTGCCCAGGTGA NGG 10 8 17 8 24 0 100 5 10