Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605133157_as0v TTTTTTTTTTTTTTTAATTCTTAGTTGTTTGG(+CAGTGT)GATCCTTAAGCATGCAAAAGCTTTGAACAGG NGG 10 8 17 8 24 0 100 5 10
    240605133155_6yam ACCCTCCTGTTCAAAGCTTTTGCATGCTTAAG(+CAGTGT)GATCCCAAACAACTAAGAATTAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605133154_7ie7 TCTACAAGCAGCTGCTCTAGCAGTAAGGGCAG(+CAGTGT)GTACCATGCTGGTGAGGAGAAAGGGGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605133153_ba72 GGCAGCCCTGCCCCTTTCTCCTCACCAGCATG(+CAGTGT)GTACCTGCCCTTACTGCTAGAGCAGCTGCTT NGG 10 8 17 8 24 0 100 5 10
    240605133151_ib5o GTCAAAATCCTTTCTTGGTTATGTGCCTCTGG(+CAGTGT)GATCCCTACTGGACACTGCGAGGGTACGTGG NGG 10 8 17 8 24 0 100 5 10
    240605133149_wqjb ATAGCCCACGTACCCTCGCAGTGTCCAGTAGG(+CAGTGT)GATCCCAGAGGCACATAACCAAGAAAGGATT NGG 10 8 17 8 24 0 100 5 10
    240605133148_ep5p TAGTAAAGCTGTCCTCCCTGCCTCTCTCACTG(+CAGTGT)GATCCGGATTGGCAACATTCCTGACTGACCC NGG 10 8 17 8 24 0 100 5 10
    240605133146_yjds TCTGAGGGTCAGTCAGGAATGTTGCCAATCCG(+CAGTGT)GATCCAGTGAGAGAGGCAGGGAGGACAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605133145_am44 CACAGCAGAACATGAATGTTCTGTATGTTTTG(+CAGTGT)GTACCCAAATCTCTAGTACACAGTACACATC NGG 10 8 17 8 24 0 100 5 10
    240605133143_5e4b GGGCAGATGTGTACTGTGTACTAGAGATTTGG(+CAGTGT)GTACCAAAACATACAGAACATTCATGTTCTG NGG 10 8 17 8 24 0 100 5 10