Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605133113_yszm TGGAATGTCTTGGAGGCCAGAGGAGGGTGTTG(+CAGTGT)GATCCCTTAGGACTGGAGTTAAAGACGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605133111_vrdc CTCACCTCTCGTCTTTAACTCCAGTCCTAAGG(+CAGTGT)GATCCAACACCCTCCTCTGGCCTCCAAGACA NGG 10 8 17 8 24 0 100 5 10
    240605133110_f8a5 ATCTAGGCTCTGGTCCTCATCTGGGGTCAAAG(+CAGTGT)GATCCCTTAGGAGGGGTCAGTTTTGCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605133108_76kk ACAATCAGAAGCAAAACTGACCCCTCCTAAGG(+CAGTGT)GATCCTTTGACCCCAGATGAGGACCAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240605133107_u50j TTTAAAGAACCACAGTGTGCAAATGTTTCAGG(+CAGTGT)GATCCTAAGCAATGCCCCAACACATGAACAG NGG 10 8 17 8 24 0 100 5 10
    240605133106_5gu8 CCAGCCTGTTCATGTGTTGGGGCATTGCTTAG(+CAGTGT)GATCCCTGAAACATTTGCACACTGTGGTTCT NGG 10 8 17 8 24 0 100 5 10
    240605133105_q1lq CCTGTAAGTACTCCCTGAGAATGCCCATTCAG(+CAGTGT)GATCCCCTGAAAGGCAAATGACAGCTATACA NGG 10 8 17 8 24 0 100 5 10
    240605133103_uijz GGTCATGTATAGCTGTCATTTGCCTTTCAGGG(+CAGTGT)GATCCTGAATGGGCATTCTCAGGGAGTACTT NGG 10 8 17 8 24 0 100 5 10
    240605133102_qeez GATTAGTTTAACTCACAGGTACTGTCTACTTG(+CAGTGT)GTACCATGCAGAAACTTTCATGCAGAATGCA NGG 10 8 17 8 24 0 100 5 10
    240605133100_q7e7 CTCTTTGCATTCTGCATGAAAGTTTCTGCATG(+CAGTGT)GTACCAAGTAGACAGTACCTGTGAGTTAAAC NGG 10 8 17 8 24 0 100 5 10