Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605133127_pi49 TTAAGCTGCAAATCTTCTCAGGTGTTCATATG(+CAGTGT)GTACCTATCCATAGATACTCACATAGTAAAA NGG 10 8 17 8 24 0 100 5 10
    240605133126_oyit TAGGGTTTTACTATGTGAGTATCTATGGATAG(+CAGTGT)GTACCATATGAACACCTGAGAAGATTTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605133124_gk0u AAGATAAGGATAAGGAAGTTGTAGTTTATATG(+CAGTGT)GATCCCAAAGTCTTGTGGTTTTGCTGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605133122_mwkx AAACTGGGAACAGCAAAACCACAAGACTTTGG(+CAGTGT)GATCCATATAAACTACAACTTCCTTATCCTT NGG 10 8 17 8 24 0 100 5 10
    240605133121_fg8e CCAGCCCTTTGGAGTAGCTCAAATCATGTGTG(+CAGTGT)GATCCCAAACATTGAAACAAGAAGCTGGAGC NGG 10 8 17 8 24 0 100 5 10
    240605133120_anwy TTAATGCTCCAGCTTCTTGTTTCAATGTTTGG(+CAGTGT)GATCCACACATGATTTGAGCTACTCCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605133118_1kib AGCACCCTTGTGTCTCTGGCTTCCTGTCACTG(+CAGTGT)GATCCAGTACCCCTCCATGCTTGGGTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240605133117_uwvl TAAAACAAGGACCCAAGCATGGAGGGGTACTG(+CAGTGT)GATCCAGTGACAGGAAGCCAGAGACACAAGG NGG 10 8 17 8 24 0 100 5 10
    240605133116_qs2m CTTAATATTTTCAAACTAATAGTTTATTATAG(+CAGTGT)GTACCATAAATCTTTAAATAAACACTATTTT NGG 10 8 17 8 24 0 100 5 10
    240605133115_stz8 AAAATAAAATAGTGTTTATTTAAAGATTTATG(+CAGTGT)GTACCTATAATAAACTATTAGTTTGAAAATA NGG 10 8 17 8 24 0 100 5 10