Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605133228_kg16 TCTTCCCACTGTGTAGTCCTGGGTCTTGTTAG(+CAGTGT)GATCCTGGCTGGGGCCTCTGGGAGCAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605133226_z0dl GCTCGACTTTTGCTCCCAGAGGCCCCAGCCAG(+CAGTGT)GATCCTAACAAGACCCAGGACTACACAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605133224_wx6s AGCAGTCTCCCAGTGCATGTTTGCATAGCTCG(+CAGTGT)GTACCAGAGCGGTTTGTAATTGAGCCTGTTT NGG 10 8 17 8 24 0 100 5 10
    240605133223_phnp ACTGCAAACAGGCTCAATTACAAACCGCTCTG(+CAGTGT)GTACCGAGCTATGCAAACATGCACTGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605133222_25xf GCTCTTTAAGGCAGAGGAGACAGACATGTGTG(+CAGTGT)GATCCGGGGCTGTTGGCTCATCTCACGTGGT NGG 10 8 17 8 24 0 100 5 10
    240605133219_0guv CTGAAACCACGTGAGATGAGCCAACAGCCCCG(+CAGTGT)GATCCACACATGTCTGTCTCCTCTGCCTTAA NGG 10 8 17 8 24 0 100 5 10
    240605133217_oz97 ATGTGTGTGTGGGTGGGTTCGTGCCTGTGTTG(+CAGTGT)GTACCTGAAGACTGAACCCAGGACCTTGTGC NGG 10 8 17 8 24 0 100 5 10
    240605133216_n6rt GGCACGCACAAGGTCCTGGGTTCAGTCTTCAG(+CAGTGT)GTACCAACACAGGCACGAACCCACCCACACA NGG 10 8 17 8 24 0 100 5 10
    240605133215_cce0 CCTTGACCAAAGATGCTGAAATACAGTGTTGG(+CAGTGT)GATCCTTGTACTCTGGAACCATGCCATCACT NGG 10 8 17 8 24 0 100 5 10
    240605133213_3zlw ACATCAGTGATGGCATGGTTCCAGAGTACAAG(+CAGTGT)GATCCCAACACTGTATTTCAGCATCTTTGGT NGG 10 8 17 8 24 0 100 5 10