Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605133330_4g35 CCCTGGGCCAAACATATTCAAACCACCATAAG(+CAGTGT)GTACCCAGAGAACAACCCCAGAACTGCACTA NGG 10 8 17 8 24 0 100 5 10
    240605133328_g3u8 TCTCATAGTGCAGTTCTGGGGTTGTTCTCTGG(+CAGTGT)GTACCTTATGGTGGTTTGAATATGTTTGGCC NGG 10 8 17 8 24 0 100 5 10
    240605133327_qilc CTTAGTCCTGAGACTGCCAGACAATCAAGCAG(+CAGTGT)GTACCCCACCAGGAGGCCAGCTCTGGACCTC NGG 10 8 17 8 24 0 100 5 10
    240605133325_5v0z TGGCAGAGGTCCAGAGCTGGCCTCCTGGTGGG(+CAGTGT)GTACCTGCTTGATTGTCTGGCAGTCTCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605133324_1evm CTTAGGGTTTTCTTTGCTCCTTTGTAAGGTGG(+CAGTGT)GATCCTCTTGCCAGTTTTCTCCAGCTGTGCC NGG 10 8 17 8 24 0 100 5 10
    240605133322_ei2s GGTGTGGCACAGCTGGAGAAAACTGGCAAGAG(+CAGTGT)GATCCCACCTTACAAAGGAGCAAAGAAAACC NGG 10 8 17 8 24 0 100 5 10
    240605133321_lrnj TCACTGAGATGTGGGACAGGGAGGGCCAGCAG(+CAGTGT)GTACCACAGGAAGAACTAGCCCATATAACTA NGG 10 8 17 8 24 0 100 5 10
    240605133319_oc0o GGCTTTAGTTATATGGGCTAGTTCTTCCTGTG(+CAGTGT)GTACCTGCTGGCCCTCCCTGTCCCACATCTC NGG 10 8 17 8 24 0 100 5 10
    240605133318_3uud GAGCCGCATCCAGTGCTCCACGAATTTAGTGG(+CAGTGT)GTACCTAGCACTGGGAGTTGAGATGAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605133316_ovrp TTTTACTTTCTCATCTCAACTCCCAGTGCTAG(+CAGTGT)GTACCCACTAAATTCGTGGAGCACTGGATGC NGG 10 8 17 8 24 0 100 5 10