Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605133404_az05 TGGGCTCCTGATTAATGAATTTCACAACAATG(+CAGTGT)GTACCCAGGCAGGAGTCCCCGTGGCTGGACC NGG 10 8 17 8 24 0 100 5 10
    240605133402_d3w0 TCAGAGGTCCAGCCACGGGGACTCCTGCCTGG(+CAGTGT)GTACCATTGTTGTGAAATTCATTAATCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605133400_dyvc CTCTAGATACAGCTGTGCCTGGAGGGCCACTG(+CAGTGT)GATCCCCACCCGTTAGATCTCGAGGGCATCA NGG 10 8 17 8 24 0 100 5 10
    240605133358_ubi8 AGACCTGATGCCCTCGAGATCTAACGGGTGGG(+CAGTGT)GATCCAGTGGCCCTCCAGGCACAGCTGTATC NGG 10 8 17 8 24 0 100 5 10
    240605133356_aao3 TCTGGACCCAAGGCTAGAATGTCTCCAGCTGG(+CAGTGT)GATCCCTGAGTTGGGCAGGCCCAACCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605133355_j3ak GCCACTGGAGGGTTGGGCCTGCCCAACTCAGG(+CAGTGT)GATCCCAGCTGGAGACATTCTAGCCTTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605133353_pl3q AATCACATTCTCTGTTCAGCCGTTAATGCTGG(+CAGTGT)GATCCATATTTAGTTTTATAAGCTTTTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605133352_oojh AAAACAGGGAAAAGCTTATAAAACTAAATATG(+CAGTGT)GATCCCAGCATTAACGGCTGAACAGAGAATG NGG 10 8 17 8 24 0 100 5 10
    240605133350_3nm6 TAGTCACTTCCCTCACCAGTGCAGGTGGGTGG(+CAGTGT)GTACCAACCATGCCTCCTCCCCCCCTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605133349_h7ut CCCCCAAAAAAGGGGGGGAGGAGGCATGGTTG(+CAGTGT)GTACCCACCCACCTGCACTGGTGAGGGAAGT NGG 10 8 17 8 24 0 100 5 10