Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605133347_zfzb GGGCAAGCCGGTTCTCTCCTTCCACCAGATGG(+CAGTGT)GATCCCAGGGGATTGAATTCAGGTTATCTGG NGG 10 8 17 8 24 0 100 5 10
    240605133344_fdxy AAATCCCAGATAACCTGAATTCAATCCCCTGG(+CAGTGT)GATCCCATCTGGTGGAAGGAGAGAACCGGCT NGG 10 8 17 8 24 0 100 5 10
    240605133342_55ok CCTGTGACCCTGCATCCCATGGGTCCTGTGAG(+CAGTGT)GTACCCGAGCCCCCTTTCTAACTCCCACCTC NGG 10 8 17 8 24 0 100 5 10
    240605133341_84si TTGGGGAGGTGGGAGTTAGAAAGGGGGCTCGG(+CAGTGT)GTACCTCACAGGACCCATGGGATGCAGGGTC NGG 10 8 17 8 24 0 100 5 10
    240605133339_s84h CTTTGTCCACTGCAGTAAATTCCACTGTGTGG(+CAGTGT)GATCCCCACAGACTTCTGGACAAGGTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240605133337_75op TGGGGAAGCCACCTTGTCCAGAAGTCTGTGGG(+CAGTGT)GATCCCACACAGTGGAATTTACTGCAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605133336_aqlg TGCCCCAGGCTCCAGAGCAAGTAGGACGTCAG(+CAGTGT)GTACCTGCTCTCTTGCCTGGCTCTAGATTTT NGG 10 8 17 8 24 0 100 5 10
    240605133335_2e7n AAATTAAAATCTAGAGCCAGGCAAGAGAGCAG(+CAGTGT)GTACCTGACGTCCTACTTGCTCTGGAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605133333_r8l4 GCCAAGTGGGACGCATTGACTGGTCACACGTG(+CAGTGT)GTACCTCTGGCCTTTCTTTTATTTATTTATT NGG 10 8 17 8 24 0 100 5 10
    240605133331_aul3 AAATAAATAAATAAATAAAAGAAAGGCCAGAG(+CAGTGT)GTACCACGTGTGACCAGTCAATGCGTCCCAC NGG 10 8 17 8 24 0 100 5 10