Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605133436_lj5j CTGAATGATGTCACCACAGCCAGGGCTCCAGG(+CAGTGT)GTACCCCCACATCCAGAGCAGCTCACAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605133434_o6nu AAAGGCAGCTGTGAGCTGCTCTGGATGTGGGG(+CAGTGT)GTACCCTGGAGCCCTGGCTGTGGTGACATCA NGG 10 8 17 8 24 0 100 5 10
    240605133433_mo6s GTTTTATTAGAAAACAAGCTAGGTGGGGAAGG(+CAGTGT)GATCCTTCCAAACCACCATGGAGGTTTGATA NGG 10 8 17 8 24 0 100 5 10
    240605133431_9yse TAAATTATCAAACCTCCATGGTGGTTTGGAAG(+CAGTGT)GATCCCTTCCCCACCTAGCTTGTTTTCTAAT NGG 10 8 17 8 24 0 100 5 10
    240605133430_d3em TTGTTCTAGAAGGCGCTCCTTTCAGGGTTGTG(+CAGTGT)GTACCTGTAGGTTAGCAGACCCTCCTCCTTT NGG 10 8 17 8 24 0 100 5 10
    240605133429_v90h GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+CAGTGT)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    240605133428_mutf CCTTCTACCAAGTGGGTAGTCCGTCCTGATAG(+CAGTGT)GTACCTTTACCTGCTGAGCCATCTGAGTAGC NGG 10 8 17 8 24 0 100 5 10
    240605133426_jutp GTGAGGCTACTCAGATGGCTCAGCAGGTAAAG(+CAGTGT)GTACCTATCAGGACGGACTACCCACTTGGTA NGG 10 8 17 8 24 0 100 5 10
    240605133425_3bp1 CAGTGGGTTGCTGCTCTCCAGGGATGTGGCTG(+CAGTGT)GTACCCCAGGTTCTTCCAGGTCATTCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605133422_di1y AGAGGCAGGAGAATGACCTGGAAGAACCTGGG(+CAGTGT)GTACCAGCCACATCCCTGGAGAGCAGCAACC NGG 10 8 17 8 24 0 100 5 10