Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605133244_4w3q GTTTTAGTTTACAAAAGAAAAATCAATGATTG(+CAGTGT)GTACCTTTTTTTAACACTCTCAGATTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605133242_allc CATATTCAGGAATCTGAGAGTGTTAAAAAAAG(+CAGTGT)GTACCAATCATTGATTTTTCTTTTGTAAACT NGG 10 8 17 8 24 0 100 5 10
    240605133240_fra8 TGAAGACAATAAATATTCCTTTGAGACCCGTG(+CAGTGT)GATCCTTCTGCGGACCCTAATGACACAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605133239_m9fn GCTCCACTTTGTGTCATTAGGGTCCGCAGAAG(+CAGTGT)GATCCACGGGTCTCAAAGGAATATTTATTGT NGG 10 8 17 8 24 0 100 5 10
    240605133237_d1qn ACCGCTCTCAAAGTAGACGGCATCGCTGCGTG(+CAGTGT)GATCCACGCCGCTCACGTAAAAGCGGCGACA NGG 10 8 17 8 24 0 100 5 10
    240605133236_pi63 GGGGTTGTCGCCGCTTTTACGTGAGCGGCGTG(+CAGTGT)GATCCACGCAGCGATGCCGTCTACTTTGAGA NGG 10 8 17 8 24 0 100 5 10
    240605133234_9r1q TCCTTTGCCTTATCTCATGGGGATGGAGGAGG(+CAGTGT)GTACCCAGGTCTCTGTTGCTTTGACTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240605133232_1f61 TTATTGAGACAGTCAAAGCAACAGAGACCTGG(+CAGTGT)GTACCCTCCTCCATCCCCATGAGATAAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605133231_x84d AGTACCTGAGGGTCTGTAGGTTGCACACTTTG(+CAGTGT)GTACCAGGTAACTTTTTTTTCTTTATAAGAA NGG 10 8 17 8 24 0 100 5 10
    240605133229_qcso ACTCTTTCTTATAAAGAAAAAAAAGTTACCTG(+CAGTGT)GTACCAAAGTGTGCAACCTACAGACCCTCAG NGG 10 8 17 8 24 0 100 5 10