Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132435_r54b TTACTTTAGCATTTTGCATTAAGATTCAAAAG(+CAGTGT)GTACCTTTTCTCTCTTTAGTGCTGTTGTAAA NGG 10 8 17 8 24 0 100 5 10
    240605132433_nrwo TAGGCTTTACAACAGCACTAAAGAGAGAAAAG(+CAGTGT)GTACCTTTTGAATCTTAATGCAAAATGCTAA NGG 10 8 17 8 24 0 100 5 10
    240605132432_3st9 GATGTTCTGGCTAATGTACACAAGGAGGTGTG(+CAGTGT)GATCCAGCAAGTGGTTTCTTCTTTGTTTTAT NGG 10 8 17 8 24 0 100 5 10
    240605132431_7x7c CAATAATAAAACAAAGAAGAAACCACTTGCTG(+CAGTGT)GATCCACACCTCCTTGTGTACATTAGCCAGA NGG 10 8 17 8 24 0 100 5 10
    240605132429_st5c ATCATGTAATTTATCTGCTCAAAACCCTCTGG(+CAGTGT)GTACCCCTATGCAGTCAAAACACTGCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605132427_jtqj CTAAAAGGAAGCAGTGTTTTGACTGCATAGGG(+CAGTGT)GTACCCAGAGGGTTTTGAGCAGATAAATTAC NGG 10 8 17 8 24 0 100 5 10
    240605132426_upkc TCATGGATGGTACTCTGGTTTTATTTGGAATG(+CAGTGT)GTACCTGATTTTCACTCACCATTATCTCATT NGG 10 8 17 8 24 0 100 5 10
    240605132425_q0hb GGCAGAATGAGATAATGGTGAGTGAAAATCAG(+CAGTGT)GTACCATTCCAAATAAAACCAGAGTACCATC NGG 10 8 17 8 24 0 100 5 10
    240605132424_nmaa CATGGCAGGTGGCTCTAAGGACACCTAGCATG(+CAGTGT)GTACCCATTTGATTAAGAGAAGAAGATGCCT NGG 10 8 17 8 24 0 100 5 10
    240605132422_f2vf CAGGAAGGCATCTTCTTCTCTTAATCAAATGG(+CAGTGT)GTACCATGCTAGGTGTCCTTAGAGCCACCTG NGG 10 8 17 8 24 0 100 5 10