Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132352_y7xd TCTACTTAATTAGCGACCAGCAAACACTAATG(+CAGTGT)GATCCATGGGGTAGGGTTTTTGGTTTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240605132350_gnhg AACAACAACAAAACCAAAAACCCTACCCCATG(+CAGTGT)GATCCATTAGTGTTTGCTGGTCGCTAATTAA NGG 10 8 17 8 24 0 100 5 10
    240605132348_0fjv GTCATCCTTGTGATGGCATGCTCCTTTGCTGG(+CAGTGT)GTACCATGTAGGGCCATTTTTTTCCCCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605132347_2eis AAACTGCAGGGGGAAAAAAATGGCCCTACATG(+CAGTGT)GTACCCAGCAAAGGAGCATGCCATCACAAGG NGG 10 8 17 8 24 0 100 5 10
    240605132345_uvg0 AAGTGCACGCATGTGCAGGAATGTCGCAGTGG(+CAGTGT)GATCCGGAGATCGCGGAGACCGTGGAGCCCG NGG 10 8 17 8 24 0 100 5 10
    240605132343_psln AGGAACGGGCTCCACGGTCTCCGCGATCTCCG(+CAGTGT)GATCCCACTGCGACATTCCTGCACATGCGTG NGG 10 8 17 8 24 0 100 5 10
    240605132342_iswh GCTGTCTTTGGGCACATCAGAAGAGGGCATTG(+CAGTGT)GATCCCATTAATGGATGGTTGTGAGCCATCT NGG 10 8 17 8 24 0 100 5 10
    240605132340_emju CCACAAGATGGCTCACAACCATCCATTAATGG(+CAGTGT)GATCCAATGCCCTCTTCTGATGTGCCCAAAG NGG 10 8 17 8 24 0 100 5 10
    240605132339_cs99 ATCACTCGCGAATTGCGTAAGCGGCCCTTCGG(+CAGTGT)GTACCCGGGCAGAGAGAACTGCGCGGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605132336_wvsa GGCAGCAAGCCCGCGCAGTTCTCTCTGCCCGG(+CAGTGT)GTACCCGAAGGGCCGCTTACGCAATTCGCGA NGG 10 8 17 8 24 0 100 5 10