Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132305_szek AAAAAGCCCTCATGACACAGAGCCTTCCTCGG(+CAGTGT)GATCCTGATCTTAGCGTTCTACTCTGAATCC NGG 10 8 17 8 24 0 100 5 10
    240605132304_30q3 CTCTGGGATTCAGAGTAGAACGCTAAGATCAG(+CAGTGT)GATCCCGAGGAAGGCTCTGTGTCATGAGGGC NGG 10 8 17 8 24 0 100 5 10
    240605132303_or2j TGTGCTCACTCCCTGGTGAACAGAGGAGCAGG(+CAGTGT)GATCCCAGAAAATAAAACGTGGAGGTCTCTT NGG 10 8 17 8 24 0 100 5 10
    240605132301_v7m9 TGGAAAAGAGACCTCCACGTTTTATTTTCTGG(+CAGTGT)GATCCCTGCTCCTCTGTTCACCAGGGAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605132300_0es1 CAGTGAGCAGCATATTTTATATACTGGTTGAG(+CAGTGT)GATCCCTAGTGAACATTATGACATCTGAACT NGG 10 8 17 8 24 0 100 5 10
    240605132258_vekm TTCATAGTTCAGATGTCATAATGTTCACTAGG(+CAGTGT)GATCCTCAACCAGTATATAAAATATGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605132257_lc0h GGGCTTGACTCTGATTGATGGCCTCTGGAGAG(+CAGTGT)GATCCTGCAGCTACCCAGATTCAAGGTTGGC NGG 10 8 17 8 24 0 100 5 10
    240605132256_99o2 TTGGAGCCAACCTTGAATCTGGGTAGCTGCAG(+CAGTGT)GATCCTCTCCAGAGGCCATCAATCAGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605132255_4pd7 ACAGGGTAATTCATTAGCAAGACATTTCATTG(+CAGTGT)GTACCGGGTTCAGGTTGTCTGGGGAATAATG NGG 10 8 17 8 24 0 100 5 10
    240605132252_j36i CCTCCCATTATTCCCCAGACAACCTGAACCCG(+CAGTGT)GTACCAATGAAATGTCTTGCTAATGAATTAC NGG 10 8 17 8 24 0 100 5 10