Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132232_oooe AAGAGATTTGAAACACATTTGAGCTGTAAGTG(+CAGTGT)GTACCTGAGGACCAGAAGATGGCCATGGGCG NGG 10 8 17 8 24 0 100 5 10
    240605132230_3un4 GAGGCCGCCCATGGCCATCTTCTGGTCCTCAG(+CAGTGT)GTACCACTTACAGCTCAAATGTGTTTCAAAT NGG 10 8 17 8 24 0 100 5 10
    240605132229_7ghk GGCCTAGTGTATCTATTATCTAGTTCTTTCGG(+CAGTGT)GTACCAGGGTTCTCCCCCTATCCTGTGTTCA NGG 10 8 17 8 24 0 100 5 10
    240605132226_5yty TGCTCTGAACACAGGATAGGGGGAGAACCCTG(+CAGTGT)GTACCCGAAAGAACTAGATAATAGATACACT NGG 10 8 17 8 24 0 100 5 10
    240605132224_59lk CTCCAGCTTCCACCTCAGAGAAAACACCAAAG(+CAGTGT)GTACCAAGAGCTAAGCCCAGGGGCCAACCAA NGG 10 8 17 8 24 0 100 5 10
    240605132223_96pa TGTTCTTGGTTGGCCCCTGGGCTTAGCTCTTG(+CAGTGT)GTACCTTTGGTGTTTTCTCTGAGGTGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240605132221_fh7y TAACTCCCAGAATCAAAAGGGCCCGAGGGAGG(+CAGTGT)GTACCTGTGAACGGTTCCCGCGGGTGGGTCT NGG 10 8 17 8 24 0 100 5 10
    240605132220_0zii TTCTTAGACCCACCCGCGGGAACCGTTCACAG(+CAGTGT)GTACCCTCCCTCGGGCCCTTTTGATTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605132219_8loy CAGGCTGGCTGCGAAAGGAGGCTACACATATG(+CAGTGT)GATCCACCTGACTGTAATCAGTGAATCTCAA NGG 10 8 17 8 24 0 100 5 10
    240605132217_oeh7 AGAAGTTGAGATTCACTGATTACAGTCAGGTG(+CAGTGT)GATCCATATGTGTAGCCTCCTTTCGCAGCCA NGG 10 8 17 8 24 0 100 5 10