Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132249_7z6t CCCCCGGGTCCAGCTCTGCTCCTGTGCCTGGG(+CAGTGT)GATCCGGAGGCTCCTGGCTGTCTTGAAATGG NGG 10 8 17 8 24 0 100 5 10
    240605132247_i29r AAAGTCCATTTCAAGACAGCCAGGAGCCTCCG(+CAGTGT)GATCCCCAGGCACAGGAGCAGAGCTGGACCC NGG 10 8 17 8 24 0 100 5 10
    240605132244_np66 AGGTGTGGCTGTAAGCTTCCAGGCTTCCCCTG(+CAGTGT)GTACCCGGCATGCCAGGGTAGCCTGCACCTG NGG 10 8 17 8 24 0 100 5 10
    240605132242_bbhj TCCTGCAGGTGCAGGCTACCCTGGCATGCCGG(+CAGTGT)GTACCAGGGGAAGCCTGGAAGCTTACAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605132241_lyyb GCACCCTGTGAGTGTAGAATGTTTTGTAAGTG(+CAGTGT)GATCCTCTTTCCTTGAAATCTGTAAGCTCAA NGG 10 8 17 8 24 0 100 5 10
    240605132240_kv33 GGACATTGAGCTTACAGATTTCAAGGAAAGAG(+CAGTGT)GATCCACTTACAAAACATTCTACACTCACAG NGG 10 8 17 8 24 0 100 5 10
    240605132238_yn0h ATCAAGTTGGTCCTACAGATGGAACATCCATG(+CAGTGT)GATCCGCAATTCAAACATGTATGAAAAATAA NGG 10 8 17 8 24 0 100 5 10
    240605132237_tuqg ATATTTTATTTTTCATACATGTTTGAATTGCG(+CAGTGT)GATCCATGGATGTTCCATCTGTAGGACCAAC NGG 10 8 17 8 24 0 100 5 10
    240605132235_8adh GGACAGACAGGAAGTACACATGAGACACCAAG(+CAGTGT)GATCCAAAACAACAGGCAGGGCTGGGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240605132233_228y GCTAATGCAGCCCAGCCCTGCCTGTTGTTTTG(+CAGTGT)GATCCTTGGTGTCTCATGTGTACTTCCTGTC NGG 10 8 17 8 24 0 100 5 10