Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132215_uabl GCTGGGGAGGCCAGTGTATCTGCTGGAGTCTG(+CAGTGT)GATCCTGACATTTACCTATTTCTTATTGGCA NGG 10 8 17 8 24 0 100 5 10
    240605132214_jb1m AGGATTGCCAATAAGAAATAGGTAAATGTCAG(+CAGTGT)GATCCAGACTCCAGCAGATACACTGGCCTCC NGG 10 8 17 8 24 0 100 5 10
    240605132212_go3h AGTCCCAACACCTATGCGTGTTCCCAACTCCG(+CAGTGT)GATCCTGTTAATACTATGGTTAAAAGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240605132211_oqar TATGGAACCACTTTTAACCATAGTATTAACAG(+CAGTGT)GATCCGGAGTTGGGAACACGCATAGGTGTTG NGG 10 8 17 8 24 0 100 5 10
    240605132209_1vfi TCTGGGCAGTAAGGACCCTATCACTGAGCCAG(+CAGTGT)GTACCTTGTCCTCTTCTTATTTTGAAGCATC NGG 10 8 17 8 24 0 100 5 10
    240605132208_5vm1 AAAATGATGCTTCAAAATAAGAAGAGGACAAG(+CAGTGT)GTACCTGGCTCAGTGATAGGGTCCTTACTGC NGG 10 8 17 8 24 0 100 5 10
    240605132207_vl0k ACAGCCAGCACAGGAAGGAGCCAGTCATTTCG(+CAGTGT)GTACCTCTGAGGTTCTGAGAGTCGAGGGATA NGG 10 8 17 8 24 0 100 5 10
    240605132205_y9un TGAGTTATCCCTCGACTCTCAGAACCTCAGAG(+CAGTGT)GTACCGAAATGACTGGCTCCTTCCTGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605132204_xnvj CTATACTCCCAGAACTTAGGATGCTGAAGGAG(+CAGTGT)GATCCAGAGTTGGAGGATAGCCTGGGCTACA NGG 10 8 17 8 24 0 100 5 10
    240605132203_kwfs CACTATGTAGCCCAGGCTATCCTCCAACTCTG(+CAGTGT)GATCCTCCTTCAGCATCCTAAGTTCTGGGAG NGG 10 8 17 8 24 0 100 5 10