Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132109_joao ACACCAGGCACACAGAGAGAAGTTCATGCCAG(+CAGTGT)GTACCAAGGGAAGATGTGAGACAGAGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605132107_qwcd AGAGGGCTGGCTCTGTCTCACATCTTCCCTTG(+CAGTGT)GTACCTGGCATGAACTTCTCTCTGTGTGCCT NGG 10 8 17 8 24 0 100 5 10
    240605132105_7soz CAGCCTAAGAATCAAGCTAAGGTATTATCAGG(+CAGTGT)GATCCCAAATGCCCCTCCCACCCAGGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605132103_05hj TAACGTCCTTCCTGGGTGGGAGGGGCATTTGG(+CAGTGT)GATCCCTGATAATACCTTAGCTTGATTCTTA NGG 10 8 17 8 24 0 100 5 10
    240605132101_k8td GGGCTCAGGCTCTTGTTGTGGGCGCTGCCTGG(+CAGTGT)GATCCATTCCTGAGGAGCTGGGCTAGATAGA NGG 10 8 17 8 24 0 100 5 10
    240605132100_ex1s GAAGGTCTATCTAGCCCAGCTCCTCAGGAATG(+CAGTGT)GATCCCAGGCAGCGCCCACAACAAGAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605132059_nvnu CACGATGCCAGGTCTGGGGCACACTGTGGTTG(+CAGTGT)GATCCAGCTGGTCACATCCAGATGGGGGAGT NGG 10 8 17 8 24 0 100 5 10
    240605132057_9bs7 ACCCCACTCCCCCATCTGGATGTGACCAGCTG(+CAGTGT)GATCCAACCACAGTGTGCCCCAGACCTGGCA NGG 10 8 17 8 24 0 100 5 10
    240605132055_vcb3 TTATGATCCCGGTGCTGAGGAGGTGGTGGCGG(+CAGTGT)GATCCCTGGAGCTCACTGGCAAGCCTCATCA NGG 10 8 17 8 24 0 100 5 10
    240605132053_woha CTCCCTGATGAGGCTTGCCAGTGAGCTCCAGG(+CAGTGT)GATCCCGCCACCACCTCCTCAGCACCGGGAT NGG 10 8 17 8 24 0 100 5 10