Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132017_aq02 AAGTGGGGAGGAATGGTACTATTGGCATGGGG(+CAGTGT)GTACCTGGTATTGAAAATGGACACATAAACT NGG 10 8 17 8 24 0 100 5 10
    240605132015_bx3e CAGCTAGTTTATGTGTCCATTTTCAATACCAG(+CAGTGT)GTACCCCCATGCCAATAGTACCATTCCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605132014_ilu1 GGGGATCTGATGTCCTCTTCTGGCCTCTGTTG(+CAGTGT)GTACCTGCACACACATGGTGTACACATACAC NGG 10 8 17 8 24 0 100 5 10
    240605132012_c74d TATTTGTGTATGTGTACACCATGTGTGTGCAG(+CAGTGT)GTACCAACAGAGGCCAGAAGAGGACATCAGA NGG 10 8 17 8 24 0 100 5 10
    240605132011_d2s3 GATTCTAGTCTGACTTGGAACAGAGGTCACAG(+CAGTGT)GTACCAACCTGATGAGACCTGCTGCTATTGT NGG 10 8 17 8 24 0 100 5 10
    240605132009_fcfm GAGACACAATAGCAGCAGGTCTCATCAGGTTG(+CAGTGT)GTACCTGTGACCTCTGTTCCAAGTCAGACTA NGG 10 8 17 8 24 0 100 5 10
    240605132008_0u4a AATACATTATAGCATGTCCTGTAGGTCTGAAG(+CAGTGT)GTACCATAGTTCAGGTACTCACATGATTTAC NGG 10 8 17 8 24 0 100 5 10
    240605132007_bjgp GGTCTGTAAATCATGTGAGTACCTGAACTATG(+CAGTGT)GTACCTTCAGACCTACAGGACATGCTATAAT NGG 10 8 17 8 24 0 100 5 10
    240605132005_pahb GGTGTCTGTGTTTGGTGGCTGATTATGGGATG(+CAGTGT)GATCCCTGGGTGGGGTAGTCTCTGGATGCAT NGG 10 8 17 8 24 0 100 5 10
    240605132002_gzst GTAAAATGCATCCAGAGACTACCCCACCCAGG(+CAGTGT)GATCCATCCCATAATCAGCCACCAAACACAG NGG 10 8 17 8 24 0 100 5 10