Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131947_qhs6 CAAAGCAGCAGAGAATAGAGGACGGAGCCCAG(+CAGTGT)GTACCTCCTCTTCCTCCTCCTCACCGTCGCC NGG 10 8 17 8 24 0 100 5 10
    240605131946_t34s ATGGAGGCGACGGTGAGGAGGAGGAAGAGGAG(+CAGTGT)GTACCTGGGCTCCGTCCTCTATTCTCTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605131944_y9k2 TGGGAAATAGACCCTGCAGTTACCTAGAATGG(+CAGTGT)GTACCAGAGAGATGATGCTGGAAGCGCCGAC NGG 10 8 17 8 24 0 100 5 10
    240605131943_cc25 ATACTGTCGGCGCTTCCAGCATCATCTCTCTG(+CAGTGT)GTACCCATTCTAGGTAACTGCAGGGTCTATT NGG 10 8 17 8 24 0 100 5 10
    240605131942_7rsc AAAACCAATTTACTGAAAAACTGCACAAGAAG(+CAGTGT)GTACCGTGAATGTAATTTCCTAGCAGGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605131941_m4g4 GCAGAGTGGCCTGCTAGGAAATTACATTCACG(+CAGTGT)GTACCTTCTTGTGCAGTTTTTCAGTAAATTG NGG 10 8 17 8 24 0 100 5 10
    240605131938_n7ro TTTTCTTTGGAGTCTGGATTTGGTGCCCGTGG(+CAGTGT)GATCCTGGAGGGCCCCACCCCCATCCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605131936_plwx AGAGACTTCAGGATGGGGGTGGGGCCCTCCAG(+CAGTGT)GATCCCACGGGCACCAAATCCAGACTCCAAA NGG 10 8 17 8 24 0 100 5 10
    240605131935_8v2t CAATCCCAGCATTCAAGAGGTAGAGGCAGGAG(+CAGTGT)GATCCGGAGTTCAAAGTCATCCTTGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605131933_3ayn CTGTTTCCAGCCAAGGATGACTTTGAACTCCG(+CAGTGT)GATCCTCCTGCCTCTACCTCTTGAATGCTGG NGG 10 8 17 8 24 0 100 5 10