Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131845_gwwf GTTAAGTTGTCTCTGTGATCCTGTGACTCTGG(+CAGTGT)GATCCTGGGACCCTGAGATCCTGGTGTGACC NGG 10 8 17 8 24 0 100 5 10
    240605131843_40ag AGCTTGGTCACACCAGGATCTCAGGGTCCCAG(+CAGTGT)GATCCCAGAGTCACAGGATCACAGAGACAAC NGG 10 8 17 8 24 0 100 5 10
    240605131841_8nio GACCGATGAGAGAGATCCGAGTGGGTTTCCTG(+CAGTGT)GATCCCACAAAAATTTCTTCATTCTTACAAA NGG 10 8 17 8 24 0 100 5 10
    240605131839_16yy TTTCTTTTGTAAGAATGAAGAAATTTTTGTGG(+CAGTGT)GATCCAGGAAACCCACTCGGATCTCTCTCAT NGG 10 8 17 8 24 0 100 5 10
    240605131838_y0q0 AACCCTATCCATGCCATGTGGGGGGATGGCAG(+CAGTGT)GATCCGTCCTGAGGAATCTGTAGCCAACAAG NGG 10 8 17 8 24 0 100 5 10
    240605131836_0xt1 TAAATCTTGTTGGCTACAGATTCCTCAGGACG(+CAGTGT)GATCCTGCCATCCCCCCACATGGCATGGATA NGG 10 8 17 8 24 0 100 5 10
    240605131835_l8hu TGTATGTTAATAAGCGAAACAAAATCATACGG(+CAGTGT)GATCCAGATACATTGCATCATTGTGTTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605131833_j9mx TAGAAATGGAACACAATGATGCAATGTATCTG(+CAGTGT)GATCCCGTATGATTTTGTTTCGCTTATTAAC NGG 10 8 17 8 24 0 100 5 10
    240605131832_fny6 ACCACATGGTGGCTCACAACCATCTGTAATGG(+CAGTGT)GATCCGATGCCCTCTTCTGGTGTGTCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605131830_t94f GCTGTCTTCTGACACACCAGAAGAGGGCATCG(+CAGTGT)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10