Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131828_a04x CCCATAGTAATGACCCTTAAAGTTGACCTCAG(+CAGTGT)GATCCACATACCAAGAAAAGAAATTGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605131827_tko9 CTCTAGCTCTCAATTTCTTTTCTTGGTATGTG(+CAGTGT)GATCCTGAGGTCAACTTTAAGGGTCATTACT NGG 10 8 17 8 24 0 100 5 10
    240605131826_y6nx ATTTTAAAATTAAGGCAGAGAGCACGAAGGAG(+CAGTGT)GTACCCAGGTCAGCCGACGGCCTCCACACAC NGG 10 8 17 8 24 0 100 5 10
    240605131823_0xf2 TGCTTGTGTGTGGAGGCCGTCGGCTGACCTGG(+CAGTGT)GTACCTCCTTCGTGCTCTCTGCCTTAATTTT NGG 10 8 17 8 24 0 100 5 10
    240605131821_npa0 TGCTCTGCCTCTCTGCTCAGGAACTAAGGATG(+CAGTGT)GTACCCTTTATCCAAAGCAGGAAGTTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605131819_2z0k GTTTCCTCACAACTTCCTGCTTTGGATAAAGG(+CAGTGT)GTACCATCCTTAGTTCCTGAGCAGAGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605131818_onvp CAAAGGTCTAGAAAGGAAGGTTGCTTGGGCTG(+CAGTGT)GATCCCTTCCCCTCCTTTTCTCCGATTAAAT NGG 10 8 17 8 24 0 100 5 10
    240605131816_yfs4 TTCGTATTTAATCGGAGAAAAGGAGGGGAAGG(+CAGTGT)GATCCAGCCCAAGCAACCTTCCTTTCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240605131814_hn3f CGCTAACACATACCCTCAGCCCCTCACTGGGG(+CAGTGT)GATCCCAAAGTGCTCTACATCTCTAGATCTT NGG 10 8 17 8 24 0 100 5 10
    240605131812_3ggd TTTGAAAGATCTAGAGATGTAGAGCACTTTGG(+CAGTGT)GATCCCCCAGTGAGGGGCTGAGGGTATGTGT NGG 10 8 17 8 24 0 100 5 10