Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131723_81ya AACTAAACTGTACAGTAAGTTCTGCAGGGGTG(+CAGTGT)GATCCCAATGTTTCTACCTTTATTTACAAAT NGG 10 8 17 8 24 0 100 5 10
    240605131721_eafd GTGATATTTGTAAATAAAGGTAGAAACATTGG(+CAGTGT)GATCCACCCCTGCAGAACTTACTGTACAGTT NGG 10 8 17 8 24 0 100 5 10
    240605131719_1pu0 AACTAAACTGTACAGTAAGTTCTGCAGGGGTG(+CAGTGT)GATCCCAATGTTTCTACCTTTATTTACAAAT NGG 10 8 17 8 24 0 100 5 10
    240605131718_1rzk GTGATATTTGTAAATAAAGGTAGAAACATTGG(+CAGTGT)GATCCACCCCTGCAGAACTTACTGTACAGTT NGG 10 8 17 8 24 0 100 5 10
    240605131716_zsc6 TTTTTTCTTCCATCCCAAGTAAAAGAGGGTTG(+CAGTGT)GATCCTGTGTGTTCCTAGCAACAGCACCATT NGG 10 8 17 8 24 0 100 5 10
    240605131715_cgdv TGAGCAATGGTGCTGTTGCTAGGAACACACAG(+CAGTGT)GATCCAACCCTCTTTTACTTGGGATGGAAGA NGG 10 8 17 8 24 0 100 5 10
    240605131714_ff8n TAACTCTCTCATGTCTGAGCAGTTCTCTTTCG(+CAGTGT)GATCCACTCTGCCTCGCTGCTGAGTCAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605131712_sm0a TTGAGTCTCTGACTCAGCAGCGAGGCAGAGTG(+CAGTGT)GATCCGAAAGAGAACTGCTCAGACATGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605131710_wnv9 AAAAAAAAAATAAATGTTTTCAAGTGTGTTGG(+CAGTGT)GTACCTAAGGGTCTTTGACAACCCCAGTACC NGG 10 8 17 8 24 0 100 5 10
    240605131709_0you GCAAAGGTACTGGGGTTGTCAAAGACCCTTAG(+CAGTGT)GTACCCAACACACTTGAAAACATTTATTTTT NGG 10 8 17 8 24 0 100 5 10