Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131622_m2ap TTCTCTGCAGCACTCAGGGAGCCCGGTAAAGG(+CAGTGT)GTACCTCCCGGAAGAATGTGGTTTTTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605131621_rxtm GGCACTTCAGAGAAAAACTAATGACAGCCTCG(+CAGTGT)GTACCTCGAACCTCATCGTTGTCTTCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605131619_j9xb AGTTGAGAAAGAAGACAACGATGAGGTTCGAG(+CAGTGT)GTACCGAGGCTGTCATTAGTTTTTCTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240605131618_yyf7 GGTTTGGAGAAAGATCCACACGTCAAAAGGTG(+CAGTGT)GTACCTGAATATGCACCAATTAGCAAACGAA NGG 10 8 17 8 24 0 100 5 10
    240605131616_01ug TTTGTTTCGTTTGCTAATTGGTGCATATTCAG(+CAGTGT)GTACCACCTTTTGACGTGTGGATCTTTCTCC NGG 10 8 17 8 24 0 100 5 10
    240605131615_m1l8 AAAAGCCCCTATGGAAAGCAAATTCCTGATTG(+CAGTGT)GTACCACCCTCGAATAGCTTATATCGTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605131614_6yci CTCTATTTAACGATATAAGCTATTCGAGGGTG(+CAGTGT)GTACCAATCAGGAATTTGCTTTCCATAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605131612_uxky GGGCCTGTTGTCCACGGAAGGCATTTGCTTAG(+CAGTGT)GATCCCCTTTGCCCCTGGCTCGGCCAGATGA NGG 10 8 17 8 24 0 100 5 10
    240605131611_1vpr ACCTTTCATCTGGCCGAGCCAGGGGCAAAGGG(+CAGTGT)GATCCTAAGCAAATGCCTTCCGTGGACAACA NGG 10 8 17 8 24 0 100 5 10
    240605131609_4dvm TTACATGATAAGAAAGCTTGACACAAGCTGAG(+CAGTGT)GTACCAAGAGGCTCCTGCCACCTGGTCCACG NGG 10 8 17 8 24 0 100 5 10