Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131707_i0ni GTATGCTGTGACCTACTTTCAGGTGTTAAACG(+CAGTGT)GATCCTGCGTAGCTTCCCTGAGCACTCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605131706_lxs8 AGAGGCAAAGAGTGCTCAGGGAAGCTACGCAG(+CAGTGT)GATCCGTTTAACACCTGAAAGTAGGTCACAG NGG 10 8 17 8 24 0 100 5 10
    240605131705_hqvj CCCTCCAGGCTGCTGATACCCACTTAGAGAGG(+CAGTGT)GATCCCTCCAGACACCCCCCCACTCTGACAC NGG 10 8 17 8 24 0 100 5 10
    240605131703_j4zd ACAAAGTGTCAGAGTGGGGGGGTGTCTGGAGG(+CAGTGT)GATCCCTCTCTAAGTGGGTATCAGCAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605131701_47ac GAGAAGAGAAAGAGATCTCGGGACGTCTGTGG(+CAGTGT)GTACCTTCATTCAGAAATACAATGGACAAGG NGG 10 8 17 8 24 0 100 5 10
    240605131700_lzm5 CCCACCCTTGTCCATTGTATTTCTGAATGAAG(+CAGTGT)GTACCCACAGACGTCCCGAGATCTCTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605131658_1v8w CTGCGCCCTGCACAGCAGACAATGTGTTCTGG(+CAGTGT)GTACCCCCAGCCTTTGCCATCTCAGGTCAAG NGG 10 8 17 8 24 0 100 5 10
    240605131656_cu5m TTTACCTTGACCTGAGATGGCAAAGGCTGGGG(+CAGTGT)GTACCCAGAACACATTGTCTGCTGTGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605131655_6saw CACCAGGTTGTGGGAAACTGGTGTAACTGTAG(+CAGTGT)GATCCATCCTAAGCAAGAACGGGAGGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240605131654_m9bj TTTTTCTTGGCCTCCCGTTCTTGCTTAGGATG(+CAGTGT)GATCCTACAGTTACACCAGTTTCCCACAACC NGG 10 8 17 8 24 0 100 5 10