Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131810_jeek CATTTCTCTCCATTTACCACGTGGCTCCTGAG(+CAGTGT)GATCCAACTCAGGTTATCAGGTAGGTGGTTA NGG 10 8 17 8 24 0 100 5 10
    240605131809_o8a6 ACACTTAACCACCTACCTGATAACCTGAGTTG(+CAGTGT)GATCCTCAGGAGCCACGTGGTAAATGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605131807_heuq CACCAGGAGCCATTCCTGGCTGCATCCCATTG(+CAGTGT)GTACCCAAATACCGGAAGCCTTGACGATGGA NGG 10 8 17 8 24 0 100 5 10
    240605131805_8z5y CCAAATCCATCGTCAAGGCTTCCGGTATTTGG(+CAGTGT)GTACCAATGGGATGCAGCCAGGAATGGCTCC NGG 10 8 17 8 24 0 100 5 10
    240605131804_e8b7 TTCTTGATCAAAGCCACTTTAGTGAGAAATAG(+CAGTGT)GTACCAGTCCTCAGGACGAGCCAGGATCCCT NGG 10 8 17 8 24 0 100 5 10
    240605131803_vmiv CCCTGAGGGATCCTGGCTCGTCCTGAGGACTG(+CAGTGT)GTACCTATTTCTCACTAAAGTGGCTTTGATC NGG 10 8 17 8 24 0 100 5 10
    240605131801_y4ss TCCATGGCCTCTGCATCAGCTCCTGCCTCCAG(+CAGTGT)GATCCTGCCCTGCTTGAGTCCTTGCCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605131759_rcxx ACTCAAGCAGGGCAAGGACTCAAGCAGGGCAG(+CAGTGT)GATCCTGGAGGCAGGAGCTGATGCAGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240605131758_jww0 GACACAGGGTGCTAAGTCTCACTGAACACTGG(+CAGTGT)GTACCCTAATAAGACTCAAACAGGCTCCATC NGG 10 8 17 8 24 0 100 5 10
    240605131756_f3cb GAGGGGATGGAGCCTGTTTGAGTCTTATTAGG(+CAGTGT)GTACCCAGTGTTCAGTGAGACTTAGCACCCT NGG 10 8 17 8 24 0 100 5 10