Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132807_fnq0 TAAAACTGTTGTGTGACATTAATAACAACAAG(+CAGTGT)GATCCTGGGAGACACAAGAAGTCAGTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240605132806_d0b6 AACACGGGCAGTCCCAGTCCGCGTGGGCGTGG(+CAGTGT)GATCCCGAGTGTGGGGAACTGTGTGGGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605132804_3yzq CCACTTGGACCCACACAGTTCCCCACACTCGG(+CAGTGT)GATCCCACGCCCACGCGGACTGGGACTGCCC NGG 10 8 17 8 24 0 100 5 10
    240605132803_balw AACACGGGCAGTCCCAGTCCGCGTGGGCGTGG(+CAGTGT)GATCCCGAGTGTGGGGAACTGTGTGGGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605132801_ryv3 CCACTTGGACCCACACAGTTCCCCACACTCGG(+CAGTGT)GATCCCACGCCCACGCGGACTGGGACTGCCC NGG 10 8 17 8 24 0 100 5 10
    240605132759_d5r0 AACACGGGCAGTCCCAGTCCGCGTGGGCGTGG(+CAGTGT)GATCCCGAGTGTGGGGAACTGTGTGGGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605132758_vpge CCACTTGGACCCACACAGTTCCCCACACTCGG(+CAGTGT)GATCCCACGCCCACGCGGACTGGGACTGCCC NGG 10 8 17 8 24 0 100 5 10
    240605132756_vi98 AACACGGGCAGTCCCAGTCCGCGTGGGCGTGG(+CAGTGT)GATCCCGAGTGTGGGGAACTGTGTGGGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605132754_4nno CCACTTGGACCCACACAGTTCCCCACACTCGG(+CAGTGT)GATCCCACGCCCACGCGGACTGGGACTGCCC NGG 10 8 17 8 24 0 100 5 10
    240605132752_dbk3 AACACGGGCAGTCCCAGTCCGCGTGGGCGTGG(+CAGTGT)GATCCCGAGTGTGGGGAACTGTGTGGGTCCA NGG 10 8 17 8 24 0 100 5 10