Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132005_pahb GGTGTCTGTGTTTGGTGGCTGATTATGGGATG(+CAGTGT)GATCCCTGGGTGGGGTAGTCTCTGGATGCAT NGG 10 8 17 8 24 0 100 5 10
    240605132002_gzst GTAAAATGCATCCAGAGACTACCCCACCCAGG(+CAGTGT)GATCCATCCCATAATCAGCCACCAAACACAG NGG 10 8 17 8 24 0 100 5 10
    240605132001_qesx TTGCACATGAGTAGAAGGACCTGAGTTTGATG(+CAGTGT)GATCCTGAGCCAGGGATGTGGCAGGAATTTA NGG 10 8 17 8 24 0 100 5 10
    240605132000_9sfo GATTATAAATTCCTGCCACATCCCTGGCTCAG(+CAGTGT)GATCCATCAAACTCAGGTCCTTCTACTCATG NGG 10 8 17 8 24 0 100 5 10
    240605131958_kke0 TTTCGCGTGGGTAGAAGGACCTGAGTTTGATG(+CAGTGT)GATCCTGAGCCAGGGATGTGGCAGGAATTTA NGG 10 8 17 8 24 0 100 5 10
    240605131957_r5ie GATTATAAATTCCTGCCACATCCCTGGCTCAG(+CAGTGT)GATCCATCAAACTCAGGTCCTTCTACCCACG NGG 10 8 17 8 24 0 100 5 10
    240605131955_n4bb CCTGACGCTTTCCACAATACACACGAGAAGGG(+CAGTGT)GTACCCCAGAGGCTAGGCTTTTCCAATGAAA NGG 10 8 17 8 24 0 100 5 10
    240605131953_tmnn AGACTTTTCATTGGAAAAGCCTAGCCTCTGGG(+CAGTGT)GTACCCCTTCTCGTGTGTATTGTGGAAAGCG NGG 10 8 17 8 24 0 100 5 10
    240605131951_5en5 GGAAGCCAGGCAGGACAGGTCCATGGGGCCTG(+CAGTGT)GTACCCACTGGCCAAAAATAACAGTGTGATT NGG 10 8 17 8 24 0 100 5 10
    240605131950_8jzm AAAAAAATCACACTGTTATTTTTGGCCAGTGG(+CAGTGT)GTACCAGGCCCCATGGACCTGTCCTGCCTGG NGG 10 8 17 8 24 0 100 5 10