Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132052_uamc TGTGGCCCTCCTGGAGCAACCTGGCAGCTACG(+CAGTGT)GATCCCAGAACCCCCTGGGCTTCAGCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240605132050_2fs0 CTCTGGGGGAGCTGAAGCCCAGGGGGTTCTGG(+CAGTGT)GATCCGTAGCTGCCAGGTTGCTCCAGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605132048_63yn CAGCACGAGGCTCGCCGTGAAGGGTGGCCTCG(+CAGTGT)GATCCTGGGCACGGCACGGTGACACTGCAAC NGG 10 8 17 8 24 0 100 5 10
    240605132046_q7md CATCTGTTGCAGTGTCACCGTGCCGTGCCCAG(+CAGTGT)GATCCGAGGCCACCCTTCACGGCGAGCCTCG NGG 10 8 17 8 24 0 100 5 10
    240605132044_cbyg TAGGGGCGCAGGGCGCGTGGAACCTAGCTGGG(+CAGTGT)GATCCAGCTTGCCTGGGTTTCTGGGGTGGTA NGG 10 8 17 8 24 0 100 5 10
    240605132043_560b CTGTTTACCACCCCAGAAACCCAGGCAAGCTG(+CAGTGT)GATCCCCAGCTAGGTTCCACGCGCCCTGCGC NGG 10 8 17 8 24 0 100 5 10
    240605132041_ji6a CCGAGTCCAGGTGCCCATGGAAGAAAGCATTG(+CAGTGT)GATCCCCCTGGAGTTGAAGTCACCTAATGTA NGG 10 8 17 8 24 0 100 5 10
    240605132039_wru2 ACATCTACATTAGGTGACTTCAACTCCAGGGG(+CAGTGT)GATCCAATGCTTTCTTCCATGGGCACCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605132037_pzej AAAAGACCCCAGAAGCACGTGGGTGAGGCCTG(+CAGTGT)GATCCAGGCACCTGTGGGAGGTGAGCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240605132035_jmvl ACATGAGGAGGCTCACCTCCCACAGGTGCCTG(+CAGTGT)GATCCAGGCCTCACCCACGTGCTTCTGGGGT NGG 10 8 17 8 24 0 100 5 10