Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132126_v26b TGGAGTCCACTGCTGGCCAGCCCCAGCACTGG(+CAGTGT)GTACCCACAGGACATGCCCCCACACACCTCA NGG 10 8 17 8 24 0 100 5 10
    240605132125_yi20 TGTTGTGAGGTGTGTGGGGGCATGTCCTGTGG(+CAGTGT)GTACCCAGTGCTGGGGCTGGCCAGCAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605132122_81kk TGTAGTCCCTCTTATCCGGCTGGATTAACCTG(+CAGTGT)GTACCCCAACATTATCCAGATTTCCTACTCC NGG 10 8 17 8 24 0 100 5 10
    240605132121_pwzc GTTTTGGAGTAGGAAATCTGGATAATGTTGGG(+CAGTGT)GTACCAGGTTAATCCAGCCGGATAAGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605132119_p7o7 CATCTCTGGAACTCGGATTCCCATCGTATTGG(+CAGTGT)GTACCACTGCTTTTAAAGCACTAAGTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240605132118_eyw9 TATGTTTAACACTTAGTGCTTTAAAAGCAGTG(+CAGTGT)GTACCCAATACGATGGGAATCCGAGTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605132117_qbxa GAGCATCCTCTGTCCTGCCTCGTGCCCAGATG(+CAGTGT)GTACCTCTGGAGAAGGCATGCGGCTGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605132115_rwdd CAGATGAGGCCAGCCGCATGCCTTCTCCAGAG(+CAGTGT)GTACCATCTGGGCACGAGGCAGGACAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240605132113_v2ao GGCCGCTCTGTTCCCCCTGACCTAGCCTCGGG(+CAGTGT)GTACCCTGTGGCCATTTGTGGTGACTTTGCC NGG 10 8 17 8 24 0 100 5 10
    240605132111_epj0 GGAGAGGCAAAGTCACCACAAATGGCCACAGG(+CAGTGT)GTACCCCGAGGCTAGGTCAGGGGGAACAGAG NGG 10 8 17 8 24 0 100 5 10