Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132142_quec CTTGGAAAGATGGGCCACATGCTGCATGACAG(+CAGTGT)GATCCTCCAAGTACCTTAACTGTTAGAGTAT NGG 10 8 17 8 24 0 100 5 10
    240605132140_kki9 CACGGATACTCTAACAGTTAAGGTACTTGGAG(+CAGTGT)GATCCTGTCATGCAGCATGTGGCCCATCTTT NGG 10 8 17 8 24 0 100 5 10
    240605132138_4rhb AGTGACCCTCCCAACACCTCTGTGCCAGCAGG(+CAGTGT)GATCCCCAAGGCAGCTACCACCTTAACATCT NGG 10 8 17 8 24 0 100 5 10
    240605132137_xfqv GTCCTAGATGTTAAGGTGGTAGCTGCCTTGGG(+CAGTGT)GATCCCTGCTGGCACAGAGGTGTTGGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605132135_1yo5 GACCTCTTCCTGTGAATGTGACCCATTCATTG(+CAGTGT)GTACCTGAAGTTATTAGTAGTATCCAATGCC NGG 10 8 17 8 24 0 100 5 10
    240605132134_m55v AACAGGGCATTGGATACTACTAATAACTTCAG(+CAGTGT)GTACCAATGAATGGGTCACATTCACAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605132133_9834 GAGAATTTTCTCTCCTCCTCCACTGGGGACAG(+CAGTGT)GATCCACCAGATGTCAATAGCGTGCTTATTT NGG 10 8 17 8 24 0 100 5 10
    240605132131_0dvj AGCGAAAATAAGCACGCTATTGACATCTGGTG(+CAGTGT)GATCCTGTCCCCAGTGGAGGAGGAGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605132130_pbks CGGCACGGCCCGCCCGGCCCCTCCACCAGGAG(+CAGTGT)GATCCGAAAGGTTACCTGTACAGTTTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605132128_96qb AGGCTACAGGAAACTGTACAGGTAACCTTTCG(+CAGTGT)GATCCTCCTGGTGGAGGGGCCGGGCGGGCCG NGG 10 8 17 8 24 0 100 5 10