Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131739_9tda TGCTGATGAAATCTGAACCATGCATCTTTTGG(+CAGTGT)GATCCACCTCCTTCAGTTTGGGGCAGATCTT NGG 10 8 17 8 24 0 100 5 10
    240605131738_gilo TCTGCAAGATCTGCCCCAAACTGAAGGAGGTG(+CAGTGT)GATCCCAAAAGATGCATGGTTCAGATTTCAT NGG 10 8 17 8 24 0 100 5 10
    240605131736_j2at CACTTTCCATCTTGTGTTAAACATTTACCCAG(+CAGTGT)GTACCTGGGTATTTTTGTTGTCAATTGGGGT NGG 10 8 17 8 24 0 100 5 10
    240605131733_slzg TGGAAACCCCAATTGACAACAAAAATACCCAG(+CAGTGT)GTACCTGGGTAAATGTTTAACACAAGATGGA NGG 10 8 17 8 24 0 100 5 10
    240605131732_2q8u AGCAAGGCCCTGTGTTCTTGACATCTGAGAGG(+CAGTGT)GATCCTACATATCAAGAGGGTCCGGGCTCCT NGG 10 8 17 8 24 0 100 5 10
    240605131731_gyu3 AGACAAGGAGCCCGGACCCTCTTGATATGTAG(+CAGTGT)GATCCCTCTCAGATGTCAAGAACACAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240605131729_w6g6 AGGTGATCCAAATGCCCTTTTGGCCCCTGCGG(+CAGTGT)GTACCACATGTATGTGGTATGTGGATCTACA NGG 10 8 17 8 24 0 100 5 10
    240605131728_rdnb CCACATGTAGATCCACATACCACATACATGTG(+CAGTGT)GTACCCGCAGGGGCCAAAAGGGCATTTGGAT NGG 10 8 17 8 24 0 100 5 10
    240605131726_3bna TCTCAATTAGTACTTATAGGGATGGGATTGCG(+CAGTGT)GTACCACGGATACTGAAGCCCTCCCTGACTC NGG 10 8 17 8 24 0 100 5 10
    240605131725_x560 GTCTAGAGTCAGGGAGGGCTTCAGTATCCGTG(+CAGTGT)GTACCGCAATCCCATCCCTATAAGTACTAAT NGG 10 8 17 8 24 0 100 5 10