Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131652_g8wg ATTTTTTTTGTTAGTTTCATCCAGATGTTCAG(+CAGTGT)GATCCAACAAATGTTATTTTCTAAACCCGCC NGG 10 8 17 8 24 0 100 5 10
    240605131651_5xk3 AGGAGGGCGGGTTTAGAAAATAACATTTGTTG(+CAGTGT)GATCCTGAACATCTGGATGAAACTAACAAAA NGG 10 8 17 8 24 0 100 5 10
    240605131650_eq5g GAAGGAGCCATGTGCCGGAGCAGCCGGAGGTG(+CAGTGT)GATCCCCACACCAGTGGCTTAGCAGGAAGGG NGG 10 8 17 8 24 0 100 5 10
    240605131648_vgg1 TCCCTCCCTTCCTGCTAAGCCACTGGTGTGGG(+CAGTGT)GATCCACCTCCGGCTGCTCCGGCACATGGCT NGG 10 8 17 8 24 0 100 5 10
    240605131646_ke05 AAACCCAACAGTATCCTGAAGAAGACAGTGTG(+CAGTGT)GATCCATGTTTAAATAAGGAGATGGGACAGG NGG 10 8 17 8 24 0 100 5 10
    240605131645_id5r ACCACCCTGTCCCATCTCCTTATTTAAACATG(+CAGTGT)GATCCACACTGTCTTCTTCAGGATACTGTTG NGG 10 8 17 8 24 0 100 5 10
    240605131643_pvtd TGCAAAGACAACAGTGTTTTAAAGTGGATTGG(+CAGTGT)GTACCAGGCTCACAAGCCTAGGTGATGTGCT NGG 10 8 17 8 24 0 100 5 10
    240605131642_17r8 ACCACAGCACATCACCTAGGCTTGTGAGCCTG(+CAGTGT)GTACCCAATCCACTTTAAAACACTGTTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605131640_54y3 AAGCATCATCCTGGTTCTCTGCCGGCTGCCAG(+CAGTGT)GTACCCTAGTGCCAAGCACGTCTGGTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605131638_8aib AGCATCAGGCACCAGACGTGCTTGGCACTAGG(+CAGTGT)GTACCTGGCAGCCGGCAGAGAACCAGGATGA NGG 10 8 17 8 24 0 100 5 10