Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131554_xey2 GTCATTTTATTGCTTTTATAAATAGACAAGGG(+CAGTGT)GATCCTCATGCCCCGTCCTTTTGTGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605131553_rjsd TGGGAATCATTGTCCAGGAGTAACTCCAGCAG(+CAGTGT)GATCCCAGGTCTCTGCAGAAGCAGGAAGGTG NGG 10 8 17 8 24 0 100 5 10
    240605131550_jqa3 CAAGCCACCTTCCTGCTTCTGCAGAGACCTGG(+CAGTGT)GATCCTGCTGGAGTTACTCCTGGACAATGAT NGG 10 8 17 8 24 0 100 5 10
    240605131549_fjvg CTCTTCCCAAAGAGCCACTTACCTCTGGACAG(+CAGTGT)GTACCCTGCGTCTGGTTCACGGTGACGATCA NGG 10 8 17 8 24 0 100 5 10
    240605131548_pmgj CAACATGATCGTCACCGTGAACCAGACGCAGG(+CAGTGT)GTACCTGTCCAGAGGTAAGTGGCTCTTTGGG NGG 10 8 17 8 24 0 100 5 10
    240605131546_cq0f TTGTATGCTGACAACTTTAATAATGTAAGGCG(+CAGTGT)GATCCGGCTCACAAGCCGGGAGGCAGCACAG NGG 10 8 17 8 24 0 100 5 10
    240605131544_iiaz CTTGTCTGTGCTGCCTCCCGGCTTGTGAGCCG(+CAGTGT)GATCCGCCTTACATTATTAAAGTTGTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605131543_l8i1 GCCAAGTGCTGCTCTGCAGTCCGTGAAGCACG(+CAGTGT)GTACCCGCATCCGGACCATCATGACTATAGG NGG 10 8 17 8 24 0 100 5 10
    240605131541_z8ph CACTCCCTATAGTCATGATGGTCCGGATGCGG(+CAGTGT)GTACCGTGCTTCACGGACTGCAGAGCAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605131539_d5ze GGTACAGCTGGATGCTGCAGAGGAGGGACTGG(+CAGTGT)GTACCATGATGGCTCCCCTAGGGAGGCAGCA NGG 10 8 17 8 24 0 100 5 10