Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131608_mr22 TCACCCGTGGACCAGGTGGCAGGAGCCTCTTG(+CAGTGT)GTACCTCAGCTTGTGTCAAGCTTTCTTATCA NGG 10 8 17 8 24 0 100 5 10
    240605131607_idyv ACCCTAAACTCAAGTCAAGGTTAATGCATGCG(+CAGTGT)GATCCCGTGACGGGGCTCAGTGAGGCCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605131605_uer6 CCCTGGCAGGGCCTCACTGAGCCCCGTCACGG(+CAGTGT)GATCCGCATGCATTAACCTTGACTTGAGTTT NGG 10 8 17 8 24 0 100 5 10
    240605131604_fxae GACTCTGGGCACGCCCTTGGCTCCCTCGGAGG(+CAGTGT)GTACCGGCTCCTCCAGAGACTAGGTTTATTT NGG 10 8 17 8 24 0 100 5 10
    240605131602_zxus GCAAAAAATAAACCTAGTCTCTGGAGGAGCCG(+CAGTGT)GTACCCTCCGAGGGAGCCAAGGGCGTGCCCA NGG 10 8 17 8 24 0 100 5 10
    240605131600_bdj8 CTAAAGGGTGGTGACTGGCTTCAGGGGGTGTG(+CAGTGT)GTACCATCTGTGCCTGCTGCCAAGACGCAGG NGG 10 8 17 8 24 0 100 5 10
    240605131559_ssmh TTGATCCTGCGTCTTGGCAGCAGGCACAGATG(+CAGTGT)GTACCACACCCCCTGAAGCCAGTCACCACCC NGG 10 8 17 8 24 0 100 5 10
    240605131558_6hks GGAAGTATAGCCAAGATAGGCCCTGGGTCATG(+CAGTGT)GTACCTGCTCACTGGAGGTCTGCAAAGTAGG NGG 10 8 17 8 24 0 100 5 10
    240605131557_jkyg CTGGGCCTACTTTGCAGACCTCCAGTGAGCAG(+CAGTGT)GTACCATGACCCAGGGCCTATCTTGGCTATA NGG 10 8 17 8 24 0 100 5 10
    240605131556_i79x TTACACTGCTTCACAAAAGGACGGGGCATGAG(+CAGTGT)GATCCCCTTGTCTATTTATAAAAGCAATAAA NGG 10 8 17 8 24 0 100 5 10