Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131523_51ky AAAACAGCATCGGCTGGGGATAAATTCCAAAG(+CAGTGT)GATCCTTTATTAAAATAGCTGAAAGAGGTCT NGG 10 8 17 8 24 0 100 5 10
    240605131521_pplu ATGTAGTTCTTGTGCACAGGTGCCACTAGCTG(+CAGTGT)GTACCCTAGGCCTAGTCCCCAACCCAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605131519_m69r CACCTCCTTTTGGGTTGGGGACTAGGCCTAGG(+CAGTGT)GTACCAGCTAGTGGCACCTGTGCACAAGAAC NGG 10 8 17 8 24 0 100 5 10
    240605131518_zoy3 ATGTATATCTCATTACCCTCATCCCCATCTTG(+CAGTGT)GTACCTGGGATCTTGGTTCTCACAAGTAATT NGG 10 8 17 8 24 0 100 5 10
    240605131515_qpdf CCTAGAATTACTTGTGAGAACCAAGATCCCAG(+CAGTGT)GTACCAAGATGGGGATGAGGGTAATGAGATA NGG 10 8 17 8 24 0 100 5 10
    240605131513_7czf CTCTTATGCTATGCATGACGATTAGCTGCCTG(+CAGTGT)GTACCTGCCCTGAGCAGGGATAAGCAGGTCA NGG 10 8 17 8 24 0 100 5 10
    240605131512_g5rn GGACTTGACCTGCTTATCCCTGCTCAGGGCAG(+CAGTGT)GTACCAGGCAGCTAATCGTCATGCATAGCAT NGG 10 8 17 8 24 0 100 5 10
    240605131510_bvc9 TGTAATGAGATCAGTTCAGCATTCACAGCCAG(+CAGTGT)GATCCCAGATCCTACTCTCTATAGCACATTG NGG 10 8 17 8 24 0 100 5 10
    240605131508_mb9s TGTGACAATGTGCTATAGAGAGTAGGATCTGG(+CAGTGT)GATCCTGGCTGTGAATGCTGAACTGATCTCA NGG 10 8 17 8 24 0 100 5 10
    240605131507_p8p5 CTGGGGGACCATAGATATGATGAAAATAAAAG(+CAGTGT)GTACCTGGAATTTTTGACACATGTAACTTTG NGG 10 8 17 8 24 0 100 5 10